摘要
用循环伏安法制备了聚L-谷氨酸修饰玻碳电极，研冤了尿酸在该电极上的电化竿行为。买验结果表明，在pH5．0的磷酸盐缓冲溶液介质中，聚L-谷氨酸修饰玻碳电极对尿酸的氧化具有良好的电催化作用，催化氧化峰电流与尿酸的浓度在2．5×10^-6～-1．0×10^-5mol／L范围内具有良好的线性关系，相关系数r=0．9943。利用该法直接测定人体尿样中尿酸的含量，回收率为98．2％-102．2％。
The preparation of poly-L-glutamic acid modified glassy carbon electrode and the electrochemical behavior of uric acid on this modified electrode were studied by cyclic voltammetry. It was found that poly-L-glutamic modified glassy carbon electrode paired with good electric catalysis action on uric acid in phosphate buffer solution （pH 5.0） , and the peak current was well -proportional to the concentration of uric acid in the rang of 2.5 × 10^ -6 - 1. 0 × 10^ -5 mol/L by cyclic vohammetry with the correlation coefficient r =0. 994 3. The procedure was utilized to determine uric acid in human urine sample with the recoveries of 98.2% -102.2%.
出处
《化学分析计量》
CAS
2007年第6期57-59,共3页
Chemical Analysis And Meterage
关键词
尿酸
聚L-谷氨酸
修饰电极
循环伏安法
uric acid, poly-L-glutamic, modified electrode, cyclic voltammetry
