摘要
目的探讨紫杉醇防治大鼠移植动脉硬化的作用及其机制。方法采用大鼠胸腹主动脉移植模型,同系移植组供、受者均为Wistar大鼠,同种移植组和紫杉醇治疗组的供者均为Wistar大鼠,受者均为SD大鼠,紫杉醇治疗组术后第1～14天腹腔注射紫杉醇2 mg·kg^(-1)·d^(-1),其它组则每日腹腔注射相同体积的生理盐水。术后30d取出移植动脉,光镜下观察移植动脉的内膜增生情况,同时采用电镜和末端脱氧核苷酸转移酶介导的三瞬酸脱氧尿嘧啶缺口末端标记法(TUNEL)检测血管平滑肌细胞(VSMCs)凋亡情况。结果术后30 d,同系移植组血管形态基本保持正常;同种移植组血管内膜明显增厚,内外膜有大量炎症细胞浸润,管腔显著狭窄;紫杉醇治疗组移植血管内膜无明显增厚,内外膜炎症细胞浸润较同种移植组明显减轻,管腔无狭窄。电镜及TUNEL检测结果显示紫杉醇治疗组VSMCs凋亡较其它两组明显增高。结论紫杉醇能明显抑制移植动脉的硬化,其作用机理可能与其诱导移植动脉VSMCs凋亡有关。
Objective To investigate the inhibitory effect of paclitaxel on rat graft arteriosclero-sis and the mechanisro. Methods The rat abdominal aortic allograft model was used. All rats were divided into three groups., isograft control group （Wistar to Wistar）, allograft group （Wistar to SD） and allograft paclitaxel-treated group （Wistar to SD）. Rats in allograft paclitaxel-treated group received paclitaxel （2mg·kg^-1·d^-1） from the operation day to post-operative day 14 and others received same dosage of vehicle （0.9% normal saline）. Animals were sacrificed and the grafts were harvested at 30th day after operation. Intimal proliferation was studied by light microscopy. The apoptosis of vascular smooth muscle cells （VSMCs） was detected by transmission electronic microscopy and terminal deoxynucleotidyl transferase biotin nick end-labeling （TUNEL） method. Results Morphological analysis showed that grafts had no change after operation in isograft control group, but in allograft group intimal proliferation, inflammatory cells infiltration in neointima and adventitia and stenosis of allografts were obvious. After treatment with paclitaxel, there was a significant decrease in intimal proliferation, inflammatory cells infiltration and stenosis. Apoptosis index of VSMCs was higher in the allograft paclitaxel-treated group than other groups. Conclusion Paclitaxel can inhibit intimal proliferation in aortic allografts and prevent the graft from arteriosclerosis possibly by inducing the apoptosis of VSMCs.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2007年第11期647-650,共4页
Chinese Journal of Organ Transplantation
基金
上海市科委重大项目(024119001)
关键词
紫杉醇
动脉硬化
血管内膜
细胞增殖
肌细胞
平滑肌
细胞凋亡
Paclitaxel
Arteriosclerosis
Tunica intima
Cell proliferation
Myocytes, smooth muscle
Apoptosis
