摘要
运用表皮实验和激光扫描共聚焦显微镜技术对NO和H2O2在根系渗透胁迫和外源脱落酸(ABA)处理诱导蚕豆气孔关闭中的作用及其相互关系进行了研究.结果表明,渗透胁迫及外源ABA处理既促进保卫细胞内源NO和H2O2形成,也诱导气孔关闭;外源H2O2和SNP可促进气孔关闭,也分别诱导保卫细胞NO和H2O2产生.还对根系渗透胁迫诱导蚕豆气孔关闭中ABA、NO和H2O2的关系进行了讨论,认为渗透胁迫可能通过ABA诱导NO和H2O2产生,促进气孔关闭且NO和H2O2之间存在相互作用.
The role and relationship of NO and H2O2 involved in the stomatal movement of Vicia faba caused by osmotic stress and exogenous abscisic acid （AlgA） were investigated by epidermal strip bioassay and laser-scanning confocal microscopy. The results showed that the generation of endogenous NO and H2O2 in guard cells and stomata closure were promoted by osmotic stress and exogenous ABA. Stomata closure and the generation of endogenous NO in guard ceils were induced by exogenous H2O2, while exogenous SNP can induce the generation of endogenous H2O2 in guard cells and stomata closure. The relationship of ABA, NO and H2O2 involved in the stomatal movement of Vicia faba caused by osmotic stress was also discussed. The generation of NO and H2O2 under osmotic stress, which indinced the stomata closure, was probably via the presence of ABA, and there was a cross talk between NO and H2O2.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第4期83-87,共5页
Journal of Shaanxi Normal University：Natural Science Edition
基金
陕西省自然科学研究计划项目(99SM20
2003C101)
关键词
渗透胁迫
脱落酸
一氧化氮
过氧化氢
气孔关闭
osmotic stress
abscisic acid
nitric oxide
hydrogen peroxide
stomata closure
