摘要
目的:探讨不同静压力对髁突软骨细胞增殖与凋亡的影响。方法:对培养到第3代新生SD大鼠髁突软骨细胞加载0、12、24、36kPa静压力1h后立即收集样本,用流式细胞仪检测细胞凋亡与增殖指数的变化。结果:随着压力的增加(0、12、24、36kPa),除24kPa外,细胞增殖指数和凋亡指数在加力结束时(0h)均减少(P<0.05),其中,细胞增殖指数在36kPa加力结束时减幅最大,细胞凋亡指数则在12kPa加力结束时减幅最大。结论:在0、12、24、36kPa力值范围内,软骨细胞增殖、凋亡与应力值存在一定的关系,但这并非是简单的线性关系。
Objective：To investigate the effects of the different static compressive stress on the proliferation and apoptosis of condylar chondrocytes in vitro. Methods： 0, 12, 24, 36 kPa static compressive stress were applied to the third passage of mandibular condylar chondrocytes for 1 hour respectively. The changes of proliferation and apoptosis of condylar chondrocytes were evaluated by flow cytometry analysis. Results： The index of proliferation and apoptosis of cells decreased with the magnitude value of static compressive stress except 24 kPa group. The most significant decrease of proliferative index and apoptosis index was found in 36 kPa group and 12 kPa group respectively. Conclusion： There might be some corelationships between magnitude of static compressive stress and the proliferation and apoptosis of condylar chondrocytes.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2007年第6期822-825,共4页
Journal of Practical Stomatology
基金
云南省科学与技术后备人才资助项目(编号:2003RC10)
关键词
髁突软骨细胞
静压力
细胞凋亡
Mandibular condylar chondrocytes
Compressive stress
Apoptosis
