摘要
目的探讨24h尿香草扁桃酸(VMA)、血清神经元特异性烯醇化酶(NSE)、血清铁蛋白(SF)、血清乳酸脱氢酶(LDH)肿瘤标志物联合检测在神经母细胞瘤(NB)诊断、分期和评价疗效中的作用。方法采用高效液相色谱仪,应用化学比色法测定24h尿VMA,采用放射免疫法检测血清NSE和SF,应用全自动生化分析仪,采用速率法测定血清LDH。分析46例NB初诊时4种肿瘤标志物阳性率、不同分期阳性率及化疗前后肿瘤标志物水平变化。结果首次入院时4种肿瘤标志物阳性率均在50%以上。其中24h尿VMA阳性率最高,达82.6%;其次为血清NSE,阳性率亦达76.1%。Ⅳ期NB血清NSE及尿VMA均较Ⅱ、Ⅲ期患儿增高。经2个疗程强化疗,4种肿瘤标志物水平均明显下降,化疗有效组与无效组比较肿瘤标志物水平下降更明显(Pa<0.01)。结论采用VMA、NSE、SF、LDH4种肿瘤标志物联合检测对NB早期诊断、分期及评价疗效有一定价值。
Objective To evaluate the significance of the combined detection of vanillylmandelic acid （VMA） in urine,hneuron-specific enolase （NSE）,ferritin （SF） and lactate dehydrogenase （LDH） in serum in the early diagnosis,staging and therapeutic evaluation in neuroblastoma（NB）.Methods Forty-six patients with NB admitted from Jan.1999 to Mar. 2005 were analyzed.Twenty-four hours urinary VMA was measured by high performance liquid chromatography,and serum NSE and SF by radioimmunoassay.LDH were measured by fully automated clinical chemistry analyzer.The positive rate at admission and different stages and the levels of the 4 tumor markers before and after chemotherapy were analyzed.Results The positive rates of the 4 tumor markers were all over 50% at admission and they were 82.6% for VMA,76.1% for NSE,63.0% for SF and 52.2% for LDH.The positive rate of VMA and NSE in stage Ⅳ were higher than those in stage Ⅱand Ⅲ.After 2 cycles of chemotherapy,the levels of 4 tumor markers were all declined,and the levels in chemotherapy-responsive group were more obvious than that in chemotherapy-resistant group （Pa〈0.01）.Conclusion The serial detection of tumor markers is valuable for the early diagnosis,staging and therapeutic response evaluation in NB.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第3期193-194,共2页
Journal of Applied Clinical Pediatrics
