摘要
经温度诱导含正向目的基因工程菌粗提液的ＤＮＡ酶活性比宿主菌本身的ＤＮＡ酶活性高７倍以上，且比等蛋白含量激发的Ｒａｊｉ细胞粗提液ＤＮＡ酶活性高２倍以上；阳性血清对重组ＥＢ病毒（ＥＢＶ）ＤＮＡ酶的中和率高达８３．５％；用重组酶粗提液和Ｒａｊｉ细胞ＤＮＡ酶检测的１０６例鼻咽癌（ＮＰＣ）病人血清抗ＥＢＶＤＮＡ酶抗体（ＥＤＡｂ）水平，结果无显著性差异，检出鼻咽癌的效率亦无显著性差异；表明重组酶经进一步提纯并标准化定量后可用于临床常规检测及大规模普查。
The activity of crude recombinant Epstein Barr virus specific DNase (rDNase) obtained from transformed E.coli. induced with changing temperature was 7 and 2 times higher than those of control group and the Raji cell DNase with same amount of protein respectively.rDNase activity could be specifically neutralizated more than 83 5% by standard sera from nasopharyngeal carcinoma patients.The EBV DNase antibody levels of 106 nasopharyngeal patients detected with rDNase and crude Raji cell DNase were coincide,indicating that this rDNase preparation could be widely used in clinical detection and general survey with its purification and standardized quantitation.
出处
《中山医科大学学报》
CSCD
1997年第3期171-173,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家教委博士点基金
关键词
基因表达
疱疹病毒4型
酶学
鼻咽肿瘤
DNA
deoxyribonucleases/analysis
gene expression
herpesvirus 4,human/enzymology
nasopharyngeal neoplasms/diagnosis
