摘要
目的:探讨血清沙眼衣原体(CT)及其热休克蛋白60(C-HSP60)抗体反应与输卵管性不孕(TFI)的关系。方法:检测了60例TFI患者(TFI组)和30例正常生育妇女(对照组)血清CT抗体。用基因工程的方法重组C-HSP60,以纯化后的C-HSP60蛋白为抗原,采用酶联免疫吸附法(ELISA),检测二组对象血清C-HSP60抗体水平。结果:TFI组和对照组血清沙眼衣原体IgG抗体阳性率分别为73.22%和43.33%,组间有显著性差异(P<0.05);TFI组C-HSP60IgG抗体阳性率为46.67%,高于对照组阳性率6.67%,差异有统计学意义(P<0.05)。TFI组中28例C-HSP60IgG抗体阳性者与32例阴性者相比,阳性者的输卵管损害程度高于阴性者(P<0.01),而阴性者的盆腔粘连程度则较阳性组严重(P<0.01)。结论:女性生殖道沙眼衣原体感染是导致TFI的重要原因,C-HSP60与其发病机理有非常密切的关系。
Objective： To investigate the prevalence of antibody to Chlamydia trachomatis and chlamydial heat shock protein 60 （C-HSP60） in women with tubal factor infertility（TFI）. Methads： Sixty women with tubal factor infertility （TFI group） and 30 women without tubal pathology （control group） were investigated to control study. An ELISA using purified recombinant C-HSP60 was developed to detect antibodies to Chlamydia trachomatis and C-HSP60 in serologic samples. Results： The positive rate of IgG antibody to Chlamydia trachomatis was 73.22% of 60 women in TFI group compared with 43.33% of 30 women in control group （P〈0.05）; C-HSP60 IgG positive rate was significantly higher in TFI group than in control group （46.67% vs 6.67%, P〈0.05）. Among the TFI group, the cases of antibody to C-HSP60 positive women were presented more tubal damage and less pelvic adhesions degree than C-HSP60 negative women （P〈0.05）. Canclusian： There is a strong association between antibody response to C-HSP60 and the development of Chlamydia trachomatis associated TFI. So C-HSP60 plays an important role in the tubal factor infertility.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2007年第11期710-713,共4页
Reproduction and Contraception
基金
深圳市科技局立项课题
项目编号:200304233
