摘要
目的:合成氧氟沙星人工抗原。方法:采用碳二亚胺法将氧氟沙星与牛血清白蛋白偶联合成完全抗原,采用混合酸酐法将氧氟沙星与卵清蛋白偶联合成应用于ELISA检测的包被抗原。结果与结论:经紫外光谱分析和动物免疫试验证明抗原合成成功。该人工抗原可用于氧氟沙星单克隆抗体的制备及其ELISA检测试剂盒的研制。
Aim :To synthesize ofloxacin artificial antigen. Methods:The immunogen,ofloxacin-BSA, was synthesized by carbodimide method; the coating antigen for ELISA,ofloxacin-OVA, was synthesized by isobutylchloroformate method. Results and Conclusion :The two artificial antigens were successfully synthesized. The synthesis of ofloxaein artificial antigen provides a basis for next monoclonal antibody preparation and ELISA kit development.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2007年第6期1086-1088,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省杰出人才创新基金资助项目0421002300
关键词
氧氟沙星
人工抗原
碳二亚胺法
混合酸酐法
ofloxacin
artificial antigen
carbodimide method
isobutylchlorofomate method