摘要
目的观察烟酸对高脂血症兔主动脉壁脂蛋白脂肪酶和三磷酸腺苷结合盒转运体A1表达的影响,探讨烟酸抗动脉粥样硬化的机制。方法10只新西兰大白兔给予高脂饮食8周后,随机分为淀粉组与烟酸组:淀粉组(n=5)饲以高脂饲料及淀粉;烟酸组(n=5)在高脂饮食基础上给予烟酸[400 mg/(kg.d)];另5只兔给予普通饮食14周作为正常对照组。14周末结束实验,采用免疫组织化学染色和半定量分析法观察烟酸对主动脉壁脂蛋白脂肪酶和三磷酸腺苷结合盒转运体A1表达的影响。结果烟酸组14周后总胆固醇、低密度脂蛋白胆固醇、甘油三酯较淀粉组分别下降43.9%、47.0%和44.3%,高密度脂蛋白胆固醇明显上升(P均<0.001);与淀粉组相比,烟酸组斑块面积减小约1.97倍,内膜厚度及内膜/中膜厚度比值分别减小约2.87倍和3.70倍;烟酸可显著下调主动脉壁脂蛋白脂肪酶的表达和上调三磷酸腺苷结合盒转运体A1的表达。结论烟酸抗动脉粥样硬化的机制可能与下调动脉壁脂蛋白脂肪酶的表达和上调三磷酸腺苷结合盒转运体A1的表达有关。
Aim To explore the effects of niacin on expression of lipoprotein lipase （LPL） and adenosine triphosphatebinding cassette transporter A1 （ABCA1） in the aorta of hypereholesterolemic rabbits and its possible antiatherogenic mechanisms. Methods Ten male New Zealand white rabbits were fed with 1% cholesterol, 7.5% protein, 8% lard diet for 8 weeks, and then were randomly divided into starch group maintained cholesterol diet and starch for 6 weeks, niacin group fed with the same cholesterol diet supplemented with niacin [400 mg/（ kg·d） ] for 6 weeks. And control group was fed with normal diet for 14 weeks. LPL and ABCA1 expression in aorta were measured by immunolfistochemistry and evaluated by semiquanfitative analysis. Results After 6-week niacin treatment, the levels of total cholesterol, low density lipoprotein cholesterol, triglycerides were decreased by 43.9%, 47.0% and 44.3% respectively, high density iipoprotein cholesterol was markedly increased （all P 〈 0.001） ; compared with starch group, the surface area of atherosclerotic lesions reduced 1.97-fold, intimal thickness and intima/ media thickness reduced 2.87-fold and 3.70-fold respectively in niacin group; niacin down-regulated LPL and up-regulated ABCA1 expression in the aorta. Conclusion The anfiatherogenic mechanisms of niacin may be related to the down-regulation of LPL and up-regulation of ABCA1 in the aorta.
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第8期626-629,共4页
Chinese Journal of Arteriosclerosis
