摘要
目的:观察壳聚糖对体外培养膀胱黏膜上皮细胞增殖的影响,探讨其治疗间质性膀胱炎的可行性。方法:获取新西兰兔膀胱黏膜,Dispase酶消化法收集上皮细胞,分不同浓度(0.3、0.6、1.2、2.4、4.8g/L)壳聚糖实验组与对照组进行细胞原代培养,免疫组织化学鉴定培养细胞。培养72h后,光镜下观察细胞生长增殖情况,NAG酶反应比色法和细胞计数法测定壳聚糖对膀胱上皮细胞增殖的影响。结果:免疫组化鉴定培养细胞为膀胱黏膜上皮细胞。与对照组相比,壳聚糖在浓度>0.3g/L时能促进兔膀胱黏膜上皮细胞的增殖,促进作用在浓度为1.2g/L时最明显(P<0.01),2.4、4.8g/L时渐降低,但仍高于对照组(P<0.01)。结论:壳聚糖体外可以促进兔膀胱黏膜上皮细胞增殖,值得进一步研究以用于治疗间质性膀胱炎。
Objective： To study the influence of chitosan on proliferation of bladder epithelial cells, so as to discuss its feasibility in treatment of interstitial cystitis. Methods： Bladder epithelial cells were harvested by enzymatic digestion of the epithelium of New-Zealand rabbit bladder. The cells were cultured in different concentrations of chitosan（0. 3,0. 6,1. 2,2. 4 and 4.8 g/L） for 72 h; untreated cells served as control. The growth and proliferation of cells were observed under microscope. The effects of chitosan on proliferation of cells were studied by NAG assay and cell counting. Results： Immunohistochemistry staining revealed that the cultured cells were epithelial cells. Chitosan （〉0.3 g/L） promoted the growth of epithelial cells, and the promoting effect was significantly when the concentration of chitosan was 1.2 g/L （P〈0.01）. The promoting effects were decreased when the concentrations of chitosan were 2.4 and 4.8 g/L,but were still higher than that of the control group（P〈0. 01 ）. Conclusion： Chitosan can promote the growth of the bladder epithelial cell in vitro, which might contribute to the treatment of interstitial cystitis
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2007年第11期1248-1251,共4页
Academic Journal of Second Military Medical University
基金
上海市自然科学基金(054119604)~~
关键词
膀胱上皮细胞
壳聚糖
细胞增殖
bladder epithelial cells
chitosan
cell proliferation
