摘要
采用ShimpackVP-ODS色谱柱(4.6mm×250mm,5μm),以甲醇-0.5%磷酸(50/50)为流动相,360nm为检测波长,用RP-HPLC法测定了笃斯越桔叶片中槲皮素的平均含量为0.78%;方法学验证结果为精密度实验RSD=1.13%(n=5),重现性实验RSD=1.73%(n=5),加样回收率平均值Y=101.6%,RSD=1.93%(n=5),说明本方法精密度、重现性、加样回收率均较好。
Determination of Quercetin in the leaves of Vacciniumu Liginosum L. was carried out. The main conditions of RPHPLC were that： the column Shimpack VP-ODS（4,6mm × 250mm, 5μm）, mobile phase methanol and 0.5% of phosphoric acid （50/50）, detecting wavelength 360 nm. The average content of quercetin was 0.78%. The results of methodology were： precision test RSD=1.13%（n=5）, reproducibility test RSD=1.73%（n=5）, average recovery rate of samples Y=101.6%, RSD=1.93% （n=5）, which indicated that the precision, reproduction and recovery rate were proper for built of determination method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第11期404-407,共4页
Food Science
基金
吉林省科技厅应用基础研究项目(20050567)
