摘要
目的建立用于测定大鼠血清中喹啉酸含量的气质联用(gas chromatography-mass spectrometry,GC-MS)技术。方法动物分为对照组和炎症模型组。经内眦静脉取血,对采集的血清样品进行纯化和衍生化,采用GC-MS联用技术测定喹啉酸含量,以全扫描方式定性,选择性离子监测(selected ion monitoring,SIM)方式定量检测。结果测定方法的专一性良好,检出限为50nmol/L,喹啉酸浓度在0.05～20μmol/L范围内线性关系良好。结论本方法检测结果准确、干扰少,检定快速,适合需要多次少量取血的动物实验应用。喹啉酸的六氟异丙醇衍生物较为稳定,但血清不宜长时间冻存,宜尽快测定。
Objective To establish a sensitive and accurate gas chromatography-mass spectrometry method for determination of quinolinic acid in rat serum. Methods The objective compound was purified and derivatized to its dihexafluoroisopropyl ester before quantitative determination by scanning method and quantitative determination by SIM method. Results The endogenous substances in serum had no interference on the measurement of quinolinic acid in the sample. Assay linearity was obtained in the range of 0.05 - 20 μmol/L with limit of quantitation （LOQ） of 0.05 μmol/L. The intraday relative standard deviation （RSD） was lower than 15%. The inter-day relative standard deviation was lower than 12%. Conclusion It is a sensitive, accurate and convenient method. The dihexafluoroisopropyl ester of quinolinic acid is quite stable, but the serum should not be preserved for a long time before derivation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第4期350-353,共4页
Journal of Third Military Medical University
