摘要
采用静态染毒法，设置50、100、150μg／L3个浓度组，研究镉、铜以及镉＋铜的联合作用24h、48h对河蚬过氧化氢酶（Catalase，（CAT））活性的影响，探讨以河蚬过氧化氢酶活性作为重金属镉、铜污染生物标记的可能性．结果显示：CAT活性的变化灵敏，且与污染物质浓度存在剂量一效应的相关关系，50～150μg／L的单独Cd、Cu作用24h、48h使河蚬过氧化氢酶的活性产生了从上升到显著下降的效应，而Cd＋Cu的联合处理则是在50～100μg/L浓度间使过氧化氢酶的活性下降，且150μg／L浓度组对CAT活性产生了极显著的抑制．结果表明，CAT活性的变化可以作为监测金属镉、铜污染的有效生物标记．
Aims of this work were to get the information concerning toxic effects of Cd and Cu on the catalase（CAT） activity in Asian clam （Corbiculafluminea） , and to evaluate the potential utility of CAT activity as biomarkers of exposure to Cd and Cu in the clam for further applications in environmental biomonitoring. In a laboratory static study, C. fluminea were exposed to three doses, 50, 100, 150 μg/L for 24 h and 48 h in single Cd, Cu and combined Cd and Cu. Results showed that CAT activity were sensitive to the pollutants and there were dose -pollutant correlativity effect for the CAT activity in the clams. The CAT activity of the clams after exposed to 50 - 150 μg/L of single Cd and Cu were from increased to remarkably decreased. Effects of 50 - 150 μg/L of combined Cd and Cu on the clams were inhibited while 150 μg/L of combined Cd and Cu were significantly inhibited. CAT activity can be used as biomarkers of level of clams exposure to Cd and Cu.
出处
《华南师范大学学报（自然科学版）》
CAS
2007年第4期81-84,97,共5页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(40373042)
广东省自然科学基金资助项目(001257)
关键词
河蚬
镉
铜
过氧化氢酶活性
Asian clam （Corbiculafluminea）
Cd
Cu
catalase （CAT） activity
