摘要
目的探讨单核细胞趋化蛋白-1(MCP-1)和巨噬细胞炎性蛋白-2(MIP-2)在重症急性胰腺炎(SAP)早期发病机制中的作用。方法以4%牛磺胆酸钠逆行胆胰管注射制作大鼠SAP模型,检测血清淀粉酶,进行胰腺组织病理学评价,采用实时定量RT-PCR方法检测各组胰腺组织中MCP-1mRNA、MIP-2mRNA表达的水平。结果SAP大鼠血清淀粉酶与假手术组比较P<0.01,随时间延长,逐步升高。胰腺组织中MCP-1mRNA、MIP-2mRNA表达高于假手术组,且表达水平与胰腺病理严重程度正相关P分别<0.05,<0.01。结论MCP-1和MIP-2在SAP早期发病机制中可能发挥重要作用。
Objective To explore the potential role of monocyte chemotactic protein-1 （MCP-1） and macrophage inflammatory protein-2 （MIP-2） in early severe acute pancreatitls （SAP）. Methods SAP was induced by retrograde injectiorl of 4% sodium taurocholate into the bilipancreatic duct. The activity of serum amylase was investigated and the pathological changes of pancreas were observed. Quantitative real-time reverse transcriptase polymerase chain reaction（QRT-PCR） method was used for detecting MCP-1 and MIP-2 expression levels in pancreatic rats in different groups. Results Compared with sham operation group, the expressions of MCP-lmRNA and MIP-2 mRNA in the pancreas were upregulated significantly in SAP group, and positively correlated with the severity of pancreas injury. Conclusion MCP-1 and MIP-2 might play important roles in the pathogenesis of early SAP.
出处
《江苏医药》
CAS
CSCD
北大核心
2007年第11期1132-1134,I0001,共4页
Jiangsu Medical Journal
