摘要
[目的]比较荧光PCR和型特异性引物PCR两种乙肝病毒基因分型方法,确立适合大规模流行病学调查和临床使用的HBV分型方法。[方法]用两种方法对福州市疾控中心保存的76份HBV DNA阳性血清标本进行基因分型。[结果]患者血清的荧光PCR法成功分型率90.8%(69/76);型特异性引物PCR法成功分型率82.9%(63/76)。两种方法均能分型的56分标本中,48份结果一致,分型结果符合率85.7%。[结论]两种分型方法的分型率类似。
[Objective] To compare the efficiency of fluorescence-PCR and multiplex-PCR method in HBV genotyping in order to find a suitable method for epidemiological survey and clinical research. [Methods] The sera from 76 HBV DNA patients were genotyped by fluorescence-PCR and multiplex -PCR method. [Results] 90.8 % （69/76） samples could be genotyped by fluorescence-PCR method and 82.8% （63/76） samples could be genotyped by multiplex-PCR method. 58 samples could genotyped by both 2 methods and 48 samples （85.7%） of them were consistent. [Conclusion] The genotyping rate is no statistical difference by fluorescence-PCR and multiplex-PCR methods.
出处
《海峡预防医学杂志》
CAS
2007年第5期14-16,共3页
Strait Journal of Preventive Medicine
基金
福州市科技局资助项目(项目编号2003-27)
