摘要
【目的】鉴定在西藏小麦地方品种中发现的特有高分子量谷蛋白亚基组合"Tibetan Dx5*+Tibetan Dy10"中的Tibetan Dy10亚基是否与普通小麦Dy10亚基为同一亚基。【方法】利用SDS-PAGE分析和TibetanDy10亚基基因的克隆和测序。【结果】表明Tibetan Dy10亚基与普通小麦中Dy10亚基广泛存在的Dx5+Dy10组合形式中的Dy10亚基的分子序列非常相似,但分别在2个六肽中的1个氨基酸部位发生替换,第335位的甘氨酸(G)和第451位的谷氨酰氨(Q)在Tibetan Dy10中均被替换为精氨酸(R)。【结论】Tibetan Dy10与普通小麦中常见的Dy10亚基基因的DNA序列存在微小差异,属于Dy10位点的一个新变异。
[ Objective ] The objective of this study was to elucidate the main difference between HMW glutenin subunit gene Tibetan Dy10 in a novel HMW gluenin subunit combination of Tibetan Dx5^＊＋Tibetan Dy10 in Tibetan wheat landrace and Dy10 in subunit combination of Dx5＋Dy10 at molecular sequence level. [Method]The Tibetan Dy10 was analyzed with SDS-PAGE analysis, DNA cloning and sequencing. [ Result] The result indicated that the molecular sequence of HMW glutenin subunit gene Tibetan Dy10 was very similar to that of Dy10 in HMW glutenin subunit combination Dx5＋Dy10. DNA sequencing analysis indicated that one amino acid in each of two hexapeptide repetitive motifs in Dy10 has been substituted in Tibetan Dy 10, respectively. The glycine （G） at position 335 and the glutamine （Q） at position 451 in the mature protein sequence of Tibetan Dy10 were replaced by arginine （R）, respectively. [Conclusion] Some minor differences were found between Tibetan Dy10 and normal Dy10, suggesting that Tibeatan Dy 10 is a new allele at Dy 10 locus.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第11期2403-2408,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金(30370882
30671272)
高等学校全国优秀博士论文基金(200458)
教育部新世纪人才支持计划(NCET-06-0819)
长江学者和创新团队发展计划(IRT0453)
国家"863"计划(2006AA10Z179和2006AA10Z1F8)
