摘要
目的研究时间分辨荧光免疫分析技术(TRFIA)在HBV感染者血清免疫学标志物检测中的应用及其与ELISA法的比较。方法用TRFIA和ELISA法对200例临床血清学标本进行检测,并对结果进行分析。结果TRFIA和ELISA法比较,HBsAg、HBeAg、抗-HBe结果符合率分别为97%、99%、94%,经配对χ2检验,P>0.05,差异无统计学意义。抗-HBe、抗-HBc结果符合率均为86%,P<0.01,差异有统计学意义。结论TRFIA与ELISA法检测乙型肝炎两对半相比,其特异性和敏感性高。TRFIA法作为一种高灵敏度的方法,其定量检测结果对HBV感染的诊断、临床分型、治疗和疗效评价都将起着十分积极的作用,为临床提供更可靠的依据。
Objective To explore the clinical value of time-resolved fluoroimmunoassay （TRFIA） in detecting HBV markers in serum and compare with enzyme linked immunosorbent assay （ELISA）. Methods TRFIA and ELISA were applied to measuring serum HBV markers for 200 cases of serum samples. The results of the two methods were analyzed. Results By comparison of two methods, the coincidence rate of HBsAg, HBeAg and anti-HBe was 97 %, 99%; and 94% respectively, and there was no statistical difference （P〉0.05）. In contrast, the coincidence rate of anti-HBs and anti-HBc was both 86% and the differences were significant （P〈0.05）. Conclusion The specificity and sensitivity of TRFIA are superior to those of ELISA. As a high sensitive assay, TRFIA contributes to doagnosis of HBV infection, clinical classification, treatment and therapeutic effect appraisal of chronic hepatitis B, and provides reliable evidence for the clinic.
出处
《检验医学与临床》
CAS
2007年第10期945-946,共2页
Laboratory Medicine and Clinic
