摘要
目的探讨严重肺挫伤及缺血再灌注后早期肺泡灌洗液（BALF）中表面活性蛋白（SP）-A、B、C、D动态变化与肺功能及肺形态学改变的关系。方法75只兔随机分为对照组（A）、肺挫伤组（B）及缺血再灌注组（C）。每组均为25只。检测各组0，1，2，3，4h肺功能变化及BAIF中SP—A、B、C、D含量百分比，进行组织病理学及电镜检查。结果与A组比较，B、C组sSP—A、B、C、D含量百分比明显减少（P〈0．01）；肺功能出现明显的损害且呈持续下降（P〈0．05）；肺泡Ⅱ型细胞电镜及病理学检查呈不同程度的炎症及变性改变。结论严重肺挫伤BALF中SP—A、B、C、D动态变化与肺功能及肺组织病理学改变一致。
Objective To study the relationship of early dynamic changes of pulmonary surfactant proteins （SP） A, B, C, and D and changes of pulmonary function and morphology in the bronchoalveolar lavage fluid （BALF） of rabbits with severe lung contusion and ischemia-reperfusion injury. Methods A total of 75 rabbits were divided randomly into 3 equal groups, ie, Group A （ control）, Group B （lung contusion） and Group C （lung contusion combined with ischemia-reperfusion injury）. The changes in pulmonary function and concentration of SPs A, B, C, and D in BALF were detected at 0, 1, 2, 3, 4 hours after lung contusion and ischemia-reperfusion injury respectively by histopathologic observation and electron microscopy. Results Compared with Group A, concentrations of SPs A, B, C, and D were significantly decreased （ P 〈 0. 01 ） and significant continual damage of lung function were found in Groups B and C （P 〈 0. 05）. Inflammation and degeneration were observed in the alveolar type Ⅱ cells of Groups B and C by electron microscopy and pathological examination. Conclusion The early dynamic changes of SPs A, B, C, and D are in consistence with the changes in pulmonary function and morphology in rabbits with severe lung contusion.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2007年第11期843-846,共4页
Chinese Journal of Trauma
基金
广东省自然科学基金资助项目（05002741）
关键词
挫伤
肺
再灌注损伤
肺表面活性剂
Contusions, lung
Reperfusion injury
Pulmonary surfactants
