摘要
以布鲁诺猕猴桃(Actinidia deliciosa cv.Bruno)成熟果实为材料,根据植物α-expansin(EXP)基因的氨基酸保守序列设计简并引物,利用RT-PCR方法结合3’RACE扩增得到1个长为957bp的cDNA片段(Ad-EXP1)。该基因片段编码211个氨基酸,与其它植物该基因核苷酸同源性为59%～85%,氨基酸同源性为73%～91%。Northern杂交结果表明,Ad-EXP1基因在采收当天的果实中表达很弱,随着果实成熟衰老进程加快趋于增强;乙酰水杨酸(ASA)处理延缓果实后熟软化和乙烯生成,也显著抑制Ad-EXP1基因表达。鉴于Ad-EXP1表达丰度与乙烯生成、果实软化程度的一致性,推测该基因在猕猴桃果实后熟软化进程中起着重要作用。
A pair of degenerate primers was designed according to the conserved amino acid sequences from EXP （α-expansin） in other plants, and an EXP cDNA fragment was obtained from ripe kiwifruit （Actinidia deliciosa cv. Bruno） by incorporating RT-PCR and 3＇ RACE. The cDNA fragment, named as Ad-EXP1, was of 957 bp in size and encodes a peptide of 211 amino acids. The fragment showed high homologous to other known EXP genes, with sequence identity at 59% to 85% and 73% to 91% for nucleotide and amino acid levels, respectively. Northern blot analysis showed that the mRNA abundance of Ad-EXP1 was low at harvest, accumulated during fruit ripening and senescence. ASA treatment significantly retarded fruit softening, inhibited ethylene production, and reduced Ad-EXP1 mRNA abundance as well. Since the expression magnitude of Ad-EXP1 was consistent with the rate of ethylene production and the degree of fruit softening, this gene might be crucial in kiwifruit ripening and softening.
出处
《果树学报》
CAS
CSCD
北大核心
2007年第6期778-782,共5页
Journal of Fruit Science
基金
国家自然科学基金(30571284)
高等学校博士学科点专项科研基金(20040335022)
