摘要
研究了碳源、氮源对彩绒革盖菌液体发酵合成漆酶的影响。结果表明,在所选的几种碳、氮源中,麸皮为试验菌株合成漆酶的较好碳源;酵母浸膏、酒石酸铵、蛋白胨均是比较理想的氮源。不同的碳氮比对彩绒革盖菌漆酶的产量有着显著的影响,高碳高氮是其生产漆酶的最佳条件。在适宜的培养条件下该菌株能合成高活力的漆酶,其酶活力可达298 U/mL以上,产酶周期为6～7 d。
This study is to investigate the influences of carbon and nitrogen sources on production of Coriolus versicolor laeease in submerged culture. The results showed that the ability of laeease seeretion was great distinet when using different earbon and nitrogen sourees. Among the seleeted earbon and nitrogen sourees in the experiment, the extraet from wheat bran was the better carbon source, and yeast extract, ammonium tartrate and peptone were the better nitrogen sourees. The laecase output was notably depended on the ratio of C/N. The high earbon and high nitrogen （HCHN） euhure was benefieial to the growth of myeelium and the synthesis of laeease. The higher laeease aetivity, up to 298 U/mL, was aehieved under the optimal eonditions and the eyele time of laeease produetion was 6 -7 days.
出处
《微生物学杂志》
CAS
CSCD
2007年第5期57-60,共4页
Journal of Microbiology
基金
江苏省教委自然科学研究基金项目(00KJB220002)
校科研基金项目(163030009)
关键词
漆酶碳源氮源液体发酵彩绒革盖菌
Laeease
Carbon sourees
Nitrogen sourecs
submerged euhure
Coriolus versicolor
