摘要
新的矮秆基因的发掘、研究和利用对水稻育种和植物生长发育机制研究有重要的作用.用60Coγ射线辐照粳稻9522,获得一个能稳定遗传的突变体.该突变体表型为株高较野生型矮,叶片短而微卷.将该突变体与籼稻广陆矮杂交,F2代呈3∶1分离,说明该突变体受隐性单基因控制.通过InDel分子标记对F2代分离群体进行遗传定位,将该基因定位于第6染色体InDel标记OS604附近.随后又发展了多对有多态性的InDel分子标记,将该基因座位精细定位在InDel标记XL6-6和XL6-1之间,AP003490和AP005619上,两个引物之间的物理距离为118 kb.本研究为该克隆基因及其作用机理的探究奠定了基础.
It is the bright road of rice breeding to study about dwarf mutant. On the other hand,dwarf mutants in rice are crucial for elucidating regulating mechanisms for plant growth and development. In the present study, the japonica cultivar 9522 was mutagenized by 60^Coγ -Ray and then a rice dwarf mutant sd-sl was found which was dwarf and had smallish,oiled leaves. Genetic analysis indicated that the phenotype was controlled by a single recessive gene. To map sd-sl locus,a F2 population was constructed from the cross between the sd-sl （japonica） and guangluai（indica）. Firstly, the sd-sl locus was roughly mapped by InDel marker, OS604, in rice chromosome 6. And then several polymorphic markers were developed for further fine-mapping. At last the sd-sl locus was mapped between the two InDel markers, XL6-6 and XL6-1. The region was delimited to about 118 kb. These results provide a basis for molecular cloning and functional analysis of sd-sl.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第6期847-851,共5页
Journal of Xiamen University：Natural Science
基金
科技部"功能基因组与生物芯片"之"水稻功能基因组"子课题(2002AA2Z1002)资助
关键词
水稻
突变体
矮秆
短-卷叶
基因定位
rice （Oryza sativa L. ）
mutant
dwarf
smallish-rolled-leaves
gene mapping
