摘要
目的:探讨硝苯地平对人牙龈成纤维细胞Ⅰ型胶原合成的影响。方法:用含浓度为108、360、1200μg/L硝苯地平的DMEM液作用体外原代培养人牙龈成纤维细胞;酶联免疫吸附试验检测细胞Ⅰ型胶原表达水平;细胞计数观察细胞增殖。结果:Ⅰ型胶原合成检测和细胞增殖试验的结果都显示在第5天,高浓度1200μg/L、360μg/L组与低浓度108μg/L之间存在显著性差异。结论:高浓度硝苯地平有促进人牙龈成纤维细胞Ⅰ型胶原合成和细胞增殖的作用。
Objective：To investigate the effect of nifedipine,a calcium channel blocker,on the expression of collagen in gingival fibroblasts in vitro. Methods：Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine （108、360、1 200 μg/L）. Enzyme-linked immunosorbent assay was used to evaluate the amount of type I collagen. Cell proliferation was measured by cell counting. Results：The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on 5 th day,especially higher in 360 μg/L and 1 200 μg/L group. Conclusion： Nifedipine can increase the expression of type Ⅰ collagen in gingival fibroblasts and cell proliferation.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期960-963,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金资助(BJ99068)
关键词
成纤维细胞
龈增生
Ⅰ型胶原
硝苯地平
fibroblast
gingival hyperplasia
type I collagen
nifedipine
