摘要
目的:研究中国恒河猴外周血中CD4+CD25+T淋巴细胞亚群及其分布频率。方法:利用流式细胞术对50只中国恒河猴外周血CD4+CD25+T淋巴细胞进行了分析。结果:发现所有被检测的恒河猴个体中均存在明显的CD4+CD25+T淋巴细胞亚群;CD4+CD25+T淋巴细胞大约占CD4+T淋巴细胞的9.1%(变化范围为2.6%～18.1%);其中CD4+CD25highT淋巴细胞约占2.5%(0.3%～5.5%)。对不同年龄和性别个体中CD4+CD25+T淋巴细胞频率的初步分析未发现统计学上有年龄或性别差异。结论:中国恒河猴可用于与CD4+CD25+T细胞相关的人类疾病的研究中。
AIM： To characterize the CD4^ ＋ CD25 ^＋T lym- phocyte subsets in the peripheral blood of Chinese rhesus macaques. METHODS CD3^＋ , CD4^＋ and CD25^＋ cells in the peripheral blood of 50 Chinese origin rhesus macaques were analyzed by three-color flow cytometry. RESULTS： It was found that all individuals had circulating CD4^＋ CD25 ^＋ T lym- phocytes. In average, 9.1% （ranging from 2.6% to 18.1% ） of CD4^＋ T cells were CD4^＋ CD25^＋ T lymphocytes. CD3 ^＋ CD4^＋ CD25^high cells represented only 2.5% （0.3% to 5.5%） of the CD4^＋ T lymphocytes. No statistically significant differences in the frequency of CD4^＋ CD25^＋ T lymphocytes or their subsets were observed between different age groups or between the male and the female rhesus macaques. CONCLU- SION： The frequency of CD4^＋ CD25^＋ T cells and CD4^＋ CD25^highT cells in the peripheral blood of Chinese rhesus macaques is similar to that in human peripheral blood, indicating that Chinese rhesus macaques may be very useful in bio-medical research on human diseases related to CD4 ^＋ CD25 ^＋ T lymphocytes.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2007年第8期734-736,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30571750)
盖茨基金异体黏膜艾滋疫苗项目(2006年)
