摘要
[目的]研究实验性矽肺大鼠在染尘后不同时间点外周血和脾组织T淋巴细胞亚群数量的变化,并探讨其意义。[方法]将60只健康雄性SD大鼠随机分为染矽尘组和对照组,每组30只。对染矽尘组以非暴露方式气管内分2次(间隔1周)分别注入1ml二氧化硅悬液(20 mg/ml)建立大鼠矽肺模型,对照组气管内注入等量的灭菌生理盐水。染尘后第7、14、21、28、35、42、49、56、63和70天各处死实验组和对照组大鼠3只,采集外周血和脾脏,用流式细胞术检测大鼠外周血、脾组织的T淋巴细胞(CD3、CD4、CD8细胞)百分率。[结果]矽肺大鼠外周血CD4水平在实验早期(第1～3周)高于对照组。在实验后期,CD3、CD4、CD4/CD8明显低于对照组(P<0.05),CD8明显高于对照组(P<0.05)。脾组织CD3水平在第1～3周明显高于对照组(P<0.05),CD8水平在第1～3周轻度增高(差异无显著性)。在实验后期,CD3、CD4、CD4/CD8明显低于对照组(P<0.05),CD8明显高于对照组(P<0.05)。[结论]在实验早期,石英增强获得性免疫反应;在实验后期,矽肺大鼠外周血及脾T淋巴细胞亚群存在明显异常,细胞免疫功能严重紊乱,石英降低了获得性免疫反应。
[ Objective ] To investigate changes and their significance of T lymphocyte subsets in peripheral blood and spleen tissue at different stages after silica exposure in rat. [ Methods ] Sixty healthy male SD rats were randomly divided into the silica exposure group and the control group. 1 ml silica suspension （ 20 mg/ml ） was administrated via intratracheal instillation twice at an interval of one week while equal amount of saline was instilled to the rats of control group. Animals were sacrificed at the 7% 14^th 21^th 28th, 35^th, 42^th, 49^th 56^th 63^rd, and 70^th day after last instillation, and then the samples of peripheral blood and spleen were collected. The levels of T lymphocyte subsets in peripheral blood and spleen were detected by flow cytometry. [ Results ] The levels of CD4 in peripheral blood of silicotic rats were higher than those in control group in early stage（ 1- 3 w ）. The levels of CD3, CD4 and the ratio of CD4/CD8 obviously decreased （ P 〈 0.05 ）, and the levels of CD8 significantly increased （ P 〈 0.05 ） in later stage of experiment. The levels of CD3 in spleen of sificotic rats significantly increased than those in control group in 1 - 3 w（ P 〈 0.05 ）, and the levels of CD8 increased slightly in 1 - 3 w（ P 〉 0.05 ）. In later stage of the experiment the levels of CD3, CD4 and the ratio of CD4/CD8 were obviously lower（ P 〈 0.05 ）, and the levels of CD8 were significantly higher than those in control group（ P 〈 0.05 ）. [ Conclusion] In the early stage of silicosis, silica increased adaptive immune responses. In the later stage, the levels of T lymphocyte subsets in peripheral blood and spleen of of silicotic rats were obviously abnormal. The function of cell immunity was in severe disorder. Silica decreased adaptive immunnity.
出处
《环境与职业医学》
CAS
北大核心
2007年第5期494-497,共4页
Journal of Environmental and Occupational Medicine
基金
安徽省优秀青年科技基金(编号:04043053)
教育部留学回国人员科研启动资金(编号:2005383)
安徽省高校省级自然科学研究重点项目(编号:KJ2007A023)
关键词
矽肺
T淋巴细胞亚群
变化
silicosis
T lymphocyte subsets
change
