摘要
目的探讨Toll样受体(TLR)和细胞因子在细菌性痢疾(以下简称"菌痢")患儿免疫应答中的作用,为该病的防治提供理论线索。方法研究对象为确诊菌痢的患儿55例,建立荧光定量PCR方法对外周血单个核细胞5种TLR和6种炎性细胞因子的基因表达进行定量检测。结果与正常对照组比较,发病在3 d以内和3 d以后的小儿外周血白细胞中TLR2、TLR4 mRNA的表达水平均有明显的升高。IL-8 mRNA的表达水平在发病的全程比对照组均明显升高,IL-12 p40 mRNA的表达水平在起病早期有3倍的明显升高。升高的TLR2、TLR4和升高的炎性细胞因子之间呈明显的正相关关系。结论菌痢患儿TLR2和TLR4显著升高,并通过促使细胞因子的产生启动并参与免疫应答。
Objective To identify the role of TLR and cytokine in immune response of children with shigellosis. Methods Blood and fecal samples were collected in acute phase of 55 shigellosis case. Real-time PCR assay was applied for the determination of mRNA expressions of 5 TLR and 6 cytokines in PBMC . Results Forty-six S. flexneri, 8 S. Sonnei and 1 S. Boydill were isolated from fecal samples. Fifty patients were diagnosed as toxemia shigellosis and 5 were common shigellosis. Both TLR2 and TLR4 mRNA levels were significantly increased in the first 3 days and 3 days later after illness onset. We also observed a 8-folded increased expression of IL-8 but 5.5-folded decrease of IFN-γ respectively in PBMC of patients during the entire period of illness. Level of IL-12 p40 was increased only at the early phase of illness onset. Patients with toxemia shigellosis have significantly higher expression levels of IL-10 and IL-12 p40 compared with patients with common shigellosis. We further demonstrated significant association between elevated levels of gene expression of TLR2 and TLR4 with IL-8 and IL- l2 m RNA. Conclusion Our results suggest that increase of TLR2 and TLR4 may modulate the immune response in the acute phase of Shigella infection and play a role in the activation of cytokines.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2007年第10期904-907,共4页
Chinese Journal of Microbiology and Immunology
