摘要
目的建立快速测定结核分枝杆菌左氧氟沙星耐药性的噬菌体生物扩增法,并探讨其在耐药性测定中的应用价值。方法应用噬菌体生物扩增法测定456株结核分枝杆菌左氧氟沙星耐药性。并与绝对浓度法结果进行比较,对不符合的菌株采用BactecMGIT960测定其最低抑菌浓度(MIC)。结果噬菌体测定456株结核分枝杆菌临床分离株左氧氟沙星敏感267株,耐药189株,绝对浓度法敏感279株,耐药177株。两法测定均为敏感257株,均为耐药167株。在32株噬菌体法与绝对浓度法测定结果不符合的菌株中30株噬菌体法与MIC测定结果相符。如以绝对浓度法药敏结果判断标准,则噬菌体法测左氧氟沙星耐药性的敏感性为94.35%,特异性为92.11%,阳性预测值为88.36%。阴性预测值为96.26%,准确性为92.98%。结论噬菌体生物扩增法测定左氧氟沙星耐药性需2d,操作简便.不需要特殊仪器设备,可作为结核分枝杆菌左氧氟沙星耐药性的快速筛选方法。
Objective To set up a rapid detection method for levofloxacin susceptibility with phage amplified biologically （PhaB） assay and to evaluate its value in the detection of levofloxacin resistance in cilnical isolates of Mycobacterium tuberculosis. Methods The assay was established to detect levofloxacin resistance in 456 clinical isolates of Mycobacterium tuberculosis, and the result was compared to that of the absolute concern tration method. The minimum inhibitory concentration （ MIC ） was detected h BACTEC-MGIT960 method for the discrepant isolates. Results Levofloxacin susceptibility results were available for 456 strains of Mycobacterium tuberculosis. A total of 189 strains were found to be levofloxacin resistant and 267 strains were levofloxacin susceptible detected by PhaB assay,but 177 and 279 strains were respectively found to be levofloxacin and resistant and susceptible by conventional methods. There were 167 and 257 levofloxacin resistant and susceptible strain beth detected by the two methods. The drug susceptibility of 30stains was the same in 32 discrepant isolates by the PhaB assay and absolute concentration method. The sensitivity, specificity, positive and negative predictive values as well as the overall accuracy for the PhaB assay were 94. 35% ,92. 11% ,88. 36% ,96. 26% and 92. 98% respectively if the judgment standard was adopted by conventional methods. Conclusion The result of PhaB assay was available within 2 days. This method,which is simple and does not need special equipment, can be used for rapid screening for levofloxacin resistance from Mycobacterium tuberculosis.
出处
《临床肺科杂志》
2007年第12期1299-1301,共3页
Journal of Clinical Pulmonary Medicine
基金
徐州市市级科研课题组研究内容
课题批准文号:XZ2006228
