摘要
目的本研究旨在观察大鼠盲肠结扎穿孔(CLP)脓毒症模型中心肌损伤的发生情况,应用血管紧张素1受体(AT1R)拮抗剂缬沙坦进行干预,观察其对心肌肾素-血管紧张素系统(RAS)包括血浆、心肌血管紧张素Ⅱ(AngⅡ)和心肌组织AT1R/AT2RmRNA表达的影响及对脓毒症大鼠心肌损伤的保护作用。方法73只Wistar大鼠制成CLP模型,随机分为手术组和手术+缬沙坦组,每组3,24,72h3个时间点,另8只为假手术对照组,观察心肌损伤指标、RAS和一氧化氮(NO)、丙二醛(MDA)等指标,观察各组各个时间点检测指标的变化。结果CLP术后,手术组血浆肌钙蛋白T(TnT)、脑钠素(BNP)、血浆和组织AngⅡ和NO、MDA浓度在3,24,72h3个时间点均明显升高,而缬沙坦组上述指标较手术未干预组明显好转。结论大鼠发生脓毒症后可出现明显的心肌损伤,表现为TnT和BNP升高。缬沙坦可通过拮抗AT1R抑制RAS过度激活及抑制NO、自由基介导的组织损伤而改善脓毒症心肌损伤。
Objective To observe the myocardial injury in rats with sepsis,the protective effect of intervention with AT1 receptor antagonist valsartan against the injury and the change of myocardial RAS system, including angiotensin Ⅱ in plasma and myocardium and the expression of AT1 / AT2 receptor mRNA in myocardium, after using valsartan. Methods Seventy-three Wistar rats were divided into surgery group and surgery＋valsartan group after cecam ligation and perforation （CLP）. Each group was further divided into three time point （ah, 24h and 72h） sub groups. Other 8 rats were used as the sham operation control group. The changes of indexes of myocardial injury, i. e. RAS system, NO and MDA,were observed. Results After CLP, troponin T（TnT）. brain natriuretic peptide （BNP） and Ag Ⅱ in plasma and myocardium, NO and MDA concentrations ascended significantly, while these indexes in valsartan group were improved significantly. Conclusions There is obvious myocardial injury in rats with sep sis, including ascent of TnT and BNP. Valsartan can improve the septic myocardial injury by inhibiting the over-activation of RAS system （by antagonizing the AT, receptor）,NO and free radicals.
出处
《中华老年多器官疾病杂志》
2007年第5期342-346,共5页
Chinese Journal of Multiple Organ Diseases in the Elderly
