摘要
目的纯化蚕蛹油中α-亚麻酸并建立含量测定方法。方法采用尿素包合法富集α-亚麻酸并用均匀设计法优化筛选其最佳工艺条件，气相色谱法测定蚕蛹油中α-亚麻酸的含量。结果尿素包舍后，蚕蛹油中α-亚麻酸的含量由35．2％提高到74、7％；Ⅱ-亚麻酸浓度在0．125～4．000mg／mL范围内线性关系良好（r=0．9999），平均回收率为99-8％。结论尿素包合法能有效提高蚕蛹油中α-亚麻酸含量；气相色谱法用于测定蚕蛹油中α-亚麻酸的含量，方法简便、准确、可靠，可用于α-亚麻酸及其制剂的质量控制。
Purpose To enriching the α-linolenie acid in silkworm pupa and to establish a determination method of its content. Methods Purifying the α-linolenie acid by urea adduet method and using uniform-design to choose the best technology condition, employing a gas chromatography （GC）-external reference method to determine the content of α-linolenie acid in silkworm pupa. Results After urea addueting, the content of α- linolenie acid in silkworm pupa increases from 35.2% to 74.7%, the linear correlation of α-linolenie acid＇s concentration from 0. 125 to 4. 000 mg/mL is fine（ r = 0. 999 9）, the average recovery is 99.8 %. Conclusion As a result urea adduetion method can enhance the content of α-linolenie acid in silkworm pupa effectively, GC-external reference method used to determine the content of α-linolenie acid in silkworm pupa is simple, exact and reliable. Therefore it can be used to control the quality of α-linolenie acid and its pharmaceutical preparations.
出处
《中国生化药物杂志》
CAS
CSCD
2007年第5期330-332,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
Α-亚麻酸
蚕蛹
尿素包合法
气相色谱
α-linolenie acid
silkworm pupa
urea adduetion fraetionation method
gas chromatography
