摘要
目的:探讨雷公藤内酯醇(triptolide,Tri)抑制体外培养的牛晶状体上皮细胞(bovine lens epithelialcell,BLECs)增殖及其机制。方法:取第4代BLECs,用MTT比色法检测增殖抑制率来测定Tri 20μg/L对细胞增殖的影响,用放射免疫法测定BLECs内cAMP浓度。结果:Tri 20μg/L,时间6,12,24,48,72 h作用在处于增殖状态BLECs,出现明显的增殖抑制作用,其增殖抑制率升高呈现明显的时间依赖性。而增殖抑制组细胞内cAMP含量明显增加。结论:Tri对晶状体上皮细胞增殖有明显的抑制作用,而该作用可能通过影响细胞内cAMP等第二信使物质浓度来完成。
Objective： To investigate the inhibitory effects on proliferation of cultured bovine-lens epithelial cells（BLECs） in vitro and the mechanism by Triptolide.Methods： The fourth generation of BLECs was used for the experimental research.The inhibitory rates of BLECs were measured by MTT（colorimetric assay）,the intracellular cAMP was measured by Radioimmunoassay.Results： The distinct inhibitory effects on the proliferation of BLECs in vitro were clearly observed and measured by Triptolide with 20 μg/L concentrations at the different time lengths.The inhibitory rates increased in the time-dependent way.The concentration levels of intracellular cAMP were evidently increased at the same time of producing inhibitory effects. Conclusion： Triptolide could effectively inhibit the proliferation of BLECs in vitro.The triptolide inhibition on the proliferation may accomplished by means of changing the concentration of the intracellular cAMP.
出处
《海南医学院学报》
CAS
2007年第5期420-421,424,427,共4页
Journal of Hainan Medical University
