摘要
目的研究血清ＨＣＶＲＮＡ与抗－ＨＣＶ之间的关系。方法对５０例抗－ＨＣＶ阳性的丙型肝炎病人，用酶标仪测血清抗－ＨＣＶＯＤ值，同时用逆转录－套式－聚合酶链反应（ＲＴ－ｎｅｓｔｅｄ－ＰＣＲ）检测血清ＨＣＶＲＮＡ。结果５０例抗－ＨＣＶ阳性丙型肝炎（ＨＣ）患者中３０例ＨＣＶＲＮＡ阳性，即６０％的ＨＣ病人存在病毒血症；在不同的抗－ＨＣＶＯＤ值范围内ＨＣＶＲＮＡ检出率不等，ＨＣＶＲＮＡ检出率随抗－ＨＣＶＯＤ值增高而增加（Ｐ＜０．０１）。结论①血清抗－ＨＣＶ与血清ＨＣＶＲＮＡ之间有较好的一致性；②抗－ＨＣＶＯＤ检测对ＨＣ的诊断及判断ＨＣ病人是否存在病毒血症具有一定价值。
Objective To study the relationship between serum HCV RNA and anti-HCV. Methods The OD value of serum anti-HCV (ELISA) in 50 hepatitis C(HC) patients was read in a photometer for enzyme-labeled assays. The serum HCV RNA was detected by RT-nested-PCR method.Results 30 of the 50 patients were positive for HCV RNA, or the rate of viremia in the HC patients was 60%。 The positive rate of HCV RNA varied with the OD value of anti-HCV proportionally(P<0.01).Conclusion [WT5”BZ]The serum level of anti-HCV is well consistent with that of HCV RNA and the determination of anti-HCV OD helps make the diagnosis of HC and judge the existence of HCV viremia.
出处
《徐州医学院学报》
CAS
1997年第1期15-16,共2页
Acta Academiae Medicinae Xuzhou
