摘要
目的分析大鼠移植心脏组织中与能量代谢相关酶的变化,探讨移植后心脏能量代谢模式的转变。方法建立大鼠心脏移植的Ono模型,采用蛋白质组学技术比较同基因移植组(供、受者均为Lewis大鼠)与异基因移植组(供者为Lewis大鼠,受者为Wistar大鼠)心脏移植后2周和8周时心脏组织中的蛋白质变化;通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)法分析获得肽质量指纹图谱,经Matrix Science查询软件搜索获得匹配的蛋白质。结果同基因移植组与异基因移植组比较,发现了65个差异表达的蛋白质,其中与心肌能量代谢相关的酶有18个。结论异基因心脏移植后心肌能量代谢发生转变,线粒体三羧酸循环发生障碍,但线粒体电子传递和ATP跨线粒体外膜的跨膜转运能力增强,糖酵解增加,脂肪酸的氧化利用增强,支链氨基酸的氧化受抑制。
Objective To analyze the changes of energy metabolism-related proteins （enzymes） and transformation of energy metabolism in transplanted heart. Methods The hearts transplanted from Lewis to Wister rats as allografts and from Lewis to Lewis rats as isografts were used to establish Ono model. The changes in proteins were observed by using proteomics technique and compared between allografts and isografts in the left ventricular myocardial tissues 2 and 8 weeks after heart transplantation. Peptide mass fingerprint map was obtained by using matrix-assisted laser desorption/ ionization time of flight mass spectrometry （MALDI-TOF-MS） and the matched proteins achieved by using Matrix Science software. Results Eighty proteins （enzymes） of total 65 differential proteins that regulated energy metabolism were identified in allografted hearts, which correlated to the Krebs cycle and electron transport system involving glycometabolism, fatty acid and branched-chain amino acids oxidation. Conclusion The energy metabolism in allografts heart was transformed： mitochondria function was decreased in Krebs cycle but enhanced in electron transport system and outer mitochondrial membrane permeability to ATP, accelerated in glycolysis and fatty acid oxidation, however, branched-chain amino acids oxidation were suppressed.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2007年第10期577-581,共5页
Chinese Journal of Organ Transplantation
基金
教育部高校博士学科点专项科研基金(200533032)
关键词
大鼠
心脏移植
能量代谢
蛋白质组
Rat
Heart transplantation
Energy metabolism
Proteome
