摘要
目的:探讨清胰汤对大鼠急性胰腺炎肺损伤时水通道蛋白-1(AQP-1)表达的影响。方法:将Wistar大鼠分为假手术组(SHAM)、肺损伤组(ALI)、地塞米松组(DEX)与清胰汤组(QYT)。采用逆行胰胆管注射去氧胆酸诱发大鼠急性胰腺炎肺损伤模型,SHAM组仅翻动胰腺,DEX组于造模后立即于股静脉注射地塞米松,QYT组于造模后立即予清胰汤灌胃治疗。各组于造模后8h取血及肺组织。检测血淀粉酶、血气、肺干/湿比值和肺组织病理切片,放免法测血清TNF-α水平,RT-PCR检测肺组织AQP-1 mRNA的表达,免疫组化法检测肺组织AQP-1的表达。结果:胰腺炎ALI组血清淀粉酶、TNF-α明显升高,DEX组与QYT组则明显下降。ALI组AQP-1mRNA和AQP-1的蛋白表达显著下调,DEX组与QYT组较肺损伤组呈显著上调。DEX组与QYT组低氧血症、肺干/湿比值、肺组织病理损害程度较ALI组明显减轻。AQP-1mRNA和AQP-1的蛋白表达与TNF-α的水平呈负相关性。结论:清胰汤通过抑制TNF-α的释放,上调水通道蛋白-1表达,从而减轻急性胰腺炎的肺损伤。
To study the influence of Qingyitang （QYT） （清胰汤） on expression of Aquaporin-l（ AQP-1 ） on acute lung injury（ALI） induced by severe acute pancreatitis（SAP） in rats Methods Adult Wistar rats were randomly divided into 4 groups： sham group, ALI group, Qingyitang group and dexamethasone（DEX） group. Sodium deoxycholate, 15g/L, was injected inversely into the common bilio-pancreatic duct in rat to make the model of ALI during SAP. Eight rats of every group were killed 8 h after making SAP model, and blood and lung tissue specimens were reserved. Serum amylase, blood gas analysis, lung tissue W/D and pulmonary homogenate were measured to evaluate the severity of pancreatitis and ALI. Serum TNF-α was also measured.RT-PCR was used to quantify AQP-1 mRNA changes and immunohistochemistry method was used for detecting AQP-1 protein changes in lung tissue. Results Compared with sham group, the lung injury were serious in ALl group as evidenced by the results of sermn amylase, blood gas analysis and pulmonary homogenate, and the expression of AQP-1 mRNA and AQP-1 protein in lung tissue were significantly decreased（ P 〈 0.01）.But in DEX group and QYT group, the expression of AQP-1 mRNA and AQP-1 protein in lung tissue was increased compared with ALl group （ P 〈 0.05）. The level of serum TNF-α was negatively correlated to the expression of AQP-1. Conclusion Qingyitang protects the lung from injury by decreasing the level of TNF-α and increasing the expression of AQP-1 in lung tissue, thus playing an important therapeutic role during ALl induced by SAP.
出处
《中国中西医结合外科杂志》
CAS
2007年第5期460-463,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(30271667)
