摘要
为利用高压静电场对柑橘类的果蔬保鲜提供一种物理处理新方法,采用自制高压静电场装置,考察不同场强度、时间和温度处理对橘青霉菌的生物学效应;用典型作用参数处理观察青霉菌芽管长度、接种于健果上的菌斑大小及孢子形态来进一步验证高压静电场对萌发率作用结果并探讨作用机理。结果表明:高压静电场对青霉菌孢子的萌发具有促进和抑制的两种完全不同的作用。场强小于40kV·m-1和处理时间小于20min能促进孢子萌发;场强大于62kV·m-1和处理时间超过60min能抑制孢子萌发,中间剂量的处理效果依据不同的处理而有所变化。温度对电场处理效果有着显著的影响,当温度升高到50℃,高剂量的场强处理可以完全抑制青霉菌孢子萌发。高压静电场对青霉菌的芽管长度、桔果上的菌斑大小的作用有着相似的结果。62kV·m-1,60min的处理使青霉菌的孢子形态结构受损,导致其孢子失去萌发能力。
In this study, P.italicum was treated with High Voltage Electrostatic Field （HVEF） in order to research its binlngieal HVEF effects and antiblastic mechanism.Spores germination rate. colony diameter on PDA and circle of spread disease on oranges were observed by changing treatment time. HVEF intensity and treatment temperature. The contfiguration of treated spore was shown by Electron microscope to research HVEF mechanism on P.italicum. The main results showed that HVEF positively increased the germination rate of P.itatlicum within the 40kV·m^-1 and 20min treatment time. However. HVEF distinctly restrained spores from germinating over 62kV·m^-1 and 60min treatment time. The treatment temperature on the ett eet of HVEF upon P.italicum was observable, especially the treatment of 50℃,62 kV·m^-1 and 60min completely restraine（l P.italicum spore from germinating. Colony diameter on PDA and circle of spread disease oranges had the same ehanging tendency. P.italicum spore was terribly destroyed with the treatment of 62kV·m^-1 and 60min.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2007年第1期118-121,共4页
Journal of Shenyang Agricultural University
基金
辽宁省教育厅课题(202050764)
关键词
高压静电场
生物效应
青霉菌
萌发率
抑菌机理
HVEF
biological effect
P.italicum
germination rate
antiblastic mechanism
