摘要
目的:改进HPLC测定人血清中盐酸左氧氟沙星浓度的方法,简化操作,减少系统误差。方法:采用反相高效液相色谱法。色谱条件:Nova-pakC18柱(4μm,3.9mm×150mm),0.04mol/L磷酸-乙腈-三乙胺(88:12:0.4,V/V,pH=3.5)为流动相,紫外检测波长为294nm,流速0.8mL/min,柱温40℃,灵敏度0.01AUFS。结果:血清样品浓度在0.125mg/L～6.0mg/L范围内,峰高对浓度外标法相关良好,回收率、精密度、稳定性、方法专属性均符合要求。经药动学验证,其体内过程符合二室模型,所有参数与文献报道值近同。结论:该方法操作简便快速、准确可靠、经济实用,可供药代动力学和生物利用度研究及临床药物监测应用。
Objective：To improve the method for determining concentration of Levofloxacin in human serum by RP-HPLC. Methods：The RP-HPLC assay was conducted using Nova-pak C18 （4μm, 3mm×150mm） with phosphoric acid-acetonitrile-triethyl amine（88：12：0.4, V/V, pH=3.5） as mobile phase at a flow rate of 0.8mL/min,The ultraviolet detection wavelength was 294nm, the column＇s temperature was 40℃, and the sensitivity was 0.01AUFS.Results：The sample＇s peak high and concentration by external standard method have the better relation.The linearity range for Levofloxacin was obtained from 0.125mg/L to 6.0mg/LThe extraction recovery, precision, stability and the specialty in this method conformed to the demand.The Levofloxacin accord with the two-compartment model by the pharmacokinetic study.All the data in the experiment was similar to the reports of other literatures.Conclusion：This method is rapid and simple, accurate and reliable, economical and practial.It is suitable for the determination of serum concentration and clinical pharmacokinetics and bioavailability.
出处
《中国药物应用与监测》
CAS
2007年第5期28-30,共3页
Chinese Journal of Drug Application and Monitoring
