摘要
目的：比较山羊痘病毒不同分离株间P32基因的同源性,并对其B细胞表位进行预测。方法：选取GenBank上10株不同山羊痘毒株P32基因序列,采用DNAsis MAX序列分析软件对该基因序列进行同源性分析,并以B细胞表位分析参数及蛋白质二级结构分析数据,综合预测P32蛋白B细胞表位。结果：10株不同山羊痘毒株P32基因核苷酸及氨基酸序列同源性分别为99.4%和98.4%;在P32蛋白的肽链中,19-60、64-80、98-118、138-182和214-275区段亲水性强,17-45、64-75、88-97、110-128、152-165和201-251区段柔韧性好,150-172、200-255区段抗原指数高,而31-59、101-119、142-145、165-172和215-252区段表面可及性高,42-56、159-181、200-208和227-259区段。结论：不同山羊痘病毒株间P32基因具有较高的同源性,P32蛋白227-251区段可能是B细胞表位优势区,为P32蛋白功能的深入研究与新型疫苗的设计提供一定的基础。
To analyze the identity matrix of P32 genes of goat pox virus isolates and to predict the B cell epitopes of this P32 protein. Methods： The P32 genes of the ten different goat pox virus were selected from GenBank to analyzed their identity matrix by the DNAsis Max analysis software, and the B cell epitopes of this P32 protein were predicted based on the analysis parameters of B cell epitopes and the characteristic of the secondary structure. Results： The P32 genes of ten strains share respectively 99.8% and 98.6% homology at nucleotide and amino acid level, and there are a stronger hydrophilicity in the regions 19 - 60, 64 - 80, 98 - 118, 138 - 182, and 214- 275, and a better flexility in 17 - 45, 64 - 75, 88 - 97, 110- 128, 152 - 165, and 201 - 251, and a higher antigenic index in 150 - 172 and 200 - 255, a finer surface proba- bility in 31 - 59, 101 - 119, 142 - 145, 165 - 172 and 215 - 252, and a spatial structure predominance in 42 - 58, 159 - 181, 200 - 208 and 227 - 259. Conclusion： The B cell epitope of P32 protein of goat pox virus was probably located or adjacent to its N- terminal region 227 - 251. Supporting that it would be a promising candidate for further research of P32 protein function and design of multiepitope- based vaccine of goat pox.
出处
《生物技术》
CAS
CSCD
2007年第5期12-14,共3页
Biotechnology
基金
贵州省优秀科技教育人才省长专项资金资助项目("以减毒细菌为载体的山羊痘基因工程粘膜疫苗的基础研究"
黔省专合字[2006]12号)
