摘要
目的XIAP相关因子1(XIAP-associated factor 1,XAF1)可与XIAP直接结合并拮抗其抗凋亡作用。本文研究了XAF1在人体肝癌组织表达,同时探讨了XAF1可能的作用机制。方法通过免疫组化方法检测了XAF1在人体肝癌组织中表达水平;建立稳定高表达XAF1的肝癌细胞克隆,通过基因芯片技术探讨了XAF1可能的作用机制。结果XAF1在人肝癌组织中表达免疫组化实验结果提示,XAF1在人肝癌组织中的表达阳性率为0.56%±0.03%,在癌旁组织中的表达阳性率为5.1±0.023%,两组均值间差异有统计学意义(P<0.05);基因芯片结果提示,7.03%的基因发生的改变有统计学意义。其中1.72%基因与细胞凋亡和细胞周期相关。在细胞凋亡和细胞周期相关基因中,41.9%与线粒体凋亡相关途径相关,可见XAF1可能通过线粒体内源性途径促进凋亡。结论XAF1在人体肝癌组织中表达水平低于癌旁组织;其抑制凋亡反应可能是通过内源性途径机制实现,XAF1有可能成为肝癌基因治疗的新靶点。
Objective To investigate the expression and mechanism of XIAP-associated factor 1 （ XAF1 ） in human liver cancer. Methods The expression of XAF1 in human liver cancer was detected hy immunohistochemical staining. The discrepancy between stahle transfectants Be17404/XAF1 and Bel7404/veetor was analyzed using gene-ehip method. Results The expression of XAF1 in human liver cancer tissue was 0.56 ± 0.03% vs 0.56 ± 0.03% in the benign nevi （P〈0.05）. The results of gene-chip analysis showed that 1.72% genes were related to apoptosis and cell cycle, 41.9% of which were related to the mitochondrial apoptosis pathway, suggesting that XAF1 probably induced apoptosis through the endogenous mitochondrial pathway. Condusions The results showed that the expression level of XAF1 in human liver cancer tissue was lower than that in benign nevi. XAF1 may induce apoptosis through the endogenous mitochondrial pathway. XAF1 may he a promising candidate for HCC gene therapy.
出处
《老年医学与保健》
CAS
2007年第5期272-274,共3页
Geriatrics & Health Care
基金
国家自然科学基金资助(编号:30100221和NO.30572142)
