摘要
目的建立测定呱安托美丁片含量的方法。方法采用HPLC法,Agilent C18色谱柱（250 mm×4.6 mm,5μm）,流动相为甲醇-乙腈-0.015 mol·L^-1KH2PO4溶液（30：30：40）（8.5%磷酸溶液调pH5.3）,检测波长313 nm,流速1.0 ml·min^-1。结果呱氨托美丁浓度10^3×103μg·ml^-1与峰面积呈良好的线性关系（r=0.9999）,平均回收率为100.02%,RSD=0.82%。结论所建方法准确、快捷、简便,不受辅料干扰,能有效地控制呱氨托美丁片的质量。
OBJECTIVE To establish an RP - HPLC method to determine the content of Amtolmetin guacil tablet. METHODS The separation was performed on a Agilent C18 column（250 mm ×4.6 mm , 5 μm）. The mobile phase was methanol - acetontrile - monopotassium phosphate buffer （0.015 mol·L^-1） （30 ： 30 ： 40, adjusted pH to 5.3 with H3PO4 ）. The detection was carried out with a uv detector at 313 nm . The flow rate was 1.0 ml·min^-1. RESULTS The linear range was 10-3×10^3 μg·ml^-1（r= 0. 9999 ） . The mean recovery was 100.02% with RSD of 0.82%. CONCLUSION This method is simple , sensitive and accurate . It can be used to effectively control the quality of Amtolmetin guacil tablet.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2007年第5期570-571,共2页
West China Journal of Pharmaceutical Sciences
