摘要
目的探讨大豆苷元对大鼠颈总动脉球囊损伤后内膜增殖的影响。方法Wistar鼠共24只,随机分为溶剂对照组、低剂量[50mg/(kg·d)]大豆苷元组和高剂量[100mg/(kg.d)]大豆苷元组,每组各8只;建立颈动脉球囊损伤动物模型。术后5周实验终点放血处死动物,取血清分别测定三酰甘油(TG)、总胆固醇(TC)、尿素氮(BUN)、丙氨酸氨基转移酶(ALT)和丙二醛(MDA);取术侧颈总动脉,切片、苏木精和伊红染色后光镜下观察血管形态并用计算机图像分析系统测量管腔面积、内膜面积和中膜面积。结果与溶剂对照组相比,低剂量组和高剂量组管腔面积均显著增加(三组分别为86893±83452μm2,199419±103352μm2和222324±56154μm2,P<0.01),内膜面积显著减少(三组分别为234370±125697μm2,147651±94731μm2和17651±9473μm2,P<0.01),内膜面积与中膜面积之比值显著降低(三组分别为172.0%±48.0%,41.3%±25.0%和6.7%±2.3%,P<0.01);三组血清生化指标之间差异无统计学意义。结论大豆苷元对大鼠颈总动脉球囊损伤后内膜增殖有一定的抑制作用。
Objective To observe the effect of daidzein on intima proliferation of rat common carotid artery injured by balloon： Methods 24 male Wistar rats were randomly assigned to 3 groups, 8 rats per group： high dose daidzein group （100 mg/kg · d）, low dose daidzein group （50 mg/kg · d） and dissolvent control group. Duplicate the rat common carotid arteries injured by balloon models. Five weeks after operation, serum biochemical indicator such as TC, TG, BUN, ALT and MDA were measured using routine biochemical methods. And pathological sections of common carotid arteries were observed and the changes of vascular histomorphology were estimated by computer analysis of photomicrograms. Results Compared with control group, the intima areas was significant decreased in high dose group and low dose group （86 893 ± 83 452 μm^2 vs 199 419 ± 103 352 μm^2 vs 222 324 ± 56 154 μm^2 , P 〈 0. 01 ）. The lumens areas was significant increased （234 370 ± 125 697 μm^2 vs 147 651 ± 94 731 μm^2 vs 17 651 ± 9 473 μm^2 ,P 〈0. 01）. The ratio of intima to media areas in was significant decreased （ 172% ± 48% vs 41.3% ± 25% vs 6. 7% ± 2. 3% ,P〈0. 01 ）. There are no significant differences in the media areas among three groups. There were no significant differences in serum TC, TG, BUN, ALT and MDA among 3 groups after 5 weeks operations （P 〉0. 05 ）. Conclusion Daidzein can inhibit the intima proliferation of Wistar rat common carotid arteries injured by balloon.
出处
《中国介入心脏病学杂志》
2007年第5期278-281,共4页
Chinese Journal of Interventional Cardiology
