摘要
以方穗山羊草为母本，普通小麦为父本杂交，并以普通小麦为父本回交１０代获得的核质杂种小麦为实验材料，测定了父母本及其核质杂种小麦ｒｕｂｉｓｃｏ的羧化活性和加氧活性。同时以方穗山羊草幼苗叶片为材料构建了库容量为５．５×１０＾５ｐｆｕ的λｇｔ１０ｃＤＮＡ文库，并以水稻ｒｂｃＳ部分片段为探针筛选该ｃＤＮＡ文库，获得了方穗山羊草ｒｂｃＳ的ｃＤＮＡ克隆ｐＲＡＳ－１。核苷酸序列分析表明该ｃＤＮＡ全长８１５ｂｐ。
The plant materials used were the leaves of Aegilops squarrosa L., Triticum aestivum L. and a nucleo-cytoplasmic hybrid wheat which was obtained by crossing T. aestivum with A. squarrosa and backcrossing 10 generations with T. aestivum as the paternal parent. The car-boxylase and oxygenase activities of these materials were assayed. At the same time, a gt10 cDNA library of 5.5x105 pfu was constructed with poly (A) RNA from the leaves of A. squarrosa seedlings. This library was screened with a partial fragment of rice rbcS, and a cDNA clone pRAS-1 that includes A. squarrosa rbcS was obtained. Nucleotide sequencing indicated that the full length of this cDNA was 815 bp. Comparison of small subunit of ribulose-l,5-bisphosphate carboxylase/ oxygenase (rubisco) from A. squarrosa with that from T. aestivum and their nucleo-cytoplasmic hybrid wheat revealed that the amino acid residues at positions 56, 84, 98 and 117 in the small sub-unit might play important roles in regulating the activities of rubisco.
基金
This wordk was supported by a grant from the National Natural Science Foundation of China.
关键词
方穗山羊草
核质杂种
小麦
RUBISCO
小亚基
Aegilops squarrosa, Nucleo-cytoplasmic hybrid wheat, Small subunit of ribulose-1,5-bisphosphate carboxylase/oxogenase
