摘要
用人乙型肝炎病毒(HHBV·)感染树鼩(Tree shrews)后,取其HBsAg及(或)HBeAg阳性的树齣血清传代感染另一批树鼩;18只树鼩分四批感染至第四代,经检测发现17只树鼩血清或肝细胞中HBV标志物阳性(94.4%);血清转氨酶(SGPT)升高,肝组织有不同程度的炎症变化。此结果表明HHBV能够在树鼩间连续传代复制,为人乙型肝炎病毒感染树鼩动物模型的建立提供又一佐证。
Primary generative infection of human hepatitis B virus(HHBV) in tree shrews had been established by the inoculation of HHBV into these animals.Thereafter,HBsAg and HBeAg positive sera obtained from the primary generative infected tree shrews were inoculated into the other tree shrews.A successful infection was continuously passaged up to four generations in 18 tree shrews.Among these tree shrews,HHBV markers were found in the sera and/or liver cells in 17(94.4%)animals;SGPT level elevated signifioant ly;different degrees of inflammation of the liver tissues were found in some animals.These results furtherly demonstrate that tree shrew may serve as an experimental animal model for studying HHBV infection.
