摘要
以福建荔枝主栽品种元红的幼胚为外植体，优化了离体培养诱导胚性愈伤组织（ＥＣ）的培养基．对ＥＣ的诱导，较高质量浓度的蔗糖（５０ｇＬ－１）和２，４－Ｄ（２～４ｍｇＬ－１）优于较低质量浓度的蔗糖（３０ｇＬ－１）和２，４－Ｄ（０．５～１．０ｍｇＬ－１）．ＢＡ和活性炭（ＡＣ）对ＥＣ的诱导有抑制作用．适当的硫代硫酸银（ＳＴＳ）（２９．４μｍｏｌＬ－１）可以抵消ＢＡ的抑制作用．在ＭＳ附加质量浓度为５０ｇＬ－１的蔗糖和２ｍｇＬ－１的２，４－Ｄ培养基上，下番枝、乌叶、元红和陈紫幼胚诱导ＥＣ的频率分别为２２％、１４．５％、１７．２％和１６．８％．在上述培养基中，元红花药产生ＥＣ的频率为０．１％～０．６％，而加入ＳＴＳ（２９．４μｍｏｌＬ－１）可提高至１．２％～５．６％．
Immature embryos of Yuanhong, one of major litchi (Litchi chinensis Sonn.) cultivars in Fujian, were used as explants to optimizing media for inducing embryogenic callus (EC). Sucrose at 50 g·L -1 and 2,4 D at 2￣4 mg·L -1 had favorable effect on inducing EC in comparison with sucrose at 30 g·L -1 and 2,4 D at 0. 5￣ 1.0 mg·L -1 . The presence of active carbon at 5 g·L -1 or BA 0.5 mg·L -1 decreased percentage of EC. However, supplementing STS (29.[KG-4]4 μmol·L -1 ) could counteract the prohibitory effect of BA. On MS basal medium supplemented with 2,4 D at 2 mg·L -1 and sucrose at 50 g·L -1 , ECs were induced from immature embryos of litchi cultivars Xiaofanzhi, Wuye, Chenzi and Yuanhong with a frequency of 22%, 14.5%, 17.2% and 16.8%, respectively. EC was also induced from anthers of Yuanhong on the medium above with a frequency of 0.1%￣0.6% and the presence of STS in this medium enhenced the frequency to 1.2%￣5.6%. Discussions were made about effect of these factors and their interactions on the induction of EC.
出处
《福建农业大学学报》
CSCD
1997年第2期168-172,共5页
Journal of Fujian Agricultural University
关键词
荔枝
胚培养
花药培养
胚胎发生
litchi
embryo culture
anther culture
embryogenesis
