摘要
为了研究高频电刺激的机理,我们设计了一个离体的细胞外电刺激培养细胞的装置,它能适用临床上外科治疗巴金森病的深部脑刺激的参数。此装置在24孔培养板相邻的两个孔中,架上钛金属丝桥;或者在10 cm直径的培养皿中,安置2条平行的钛金属丝。我们证明它在GH3和PC12细胞的培养中无毒性。初步的实验结果显示它适用于离体细胞新陈代谢变化的实验,基因芯片及蛋白芯片等技术的实验。
In order to study the mechanisms of high frequency stimulation （ HFS）, we designed an in vitro device to deliver extracellular electrical stimulation to live cells in culture, using the same parameters as in the surgical treatment of Parkinson＇s disease （PD）. The system was made of a 24 wells culture plate electrified with titanium wire bridges or two titanium wires placed parallel in a culture dish of 10 centimetres diameter. The extremities of the series of wells were connected to a constant current generator. The result showed that this system had no toxicity on GH3 and PC12 cell cultures. Preliminary experiences demonstrate that this system is suitable for the study with DNA and protein chip technologies.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2007年第5期489-494,共6页
Chinese Journal of Neuroanatomy
基金
法国国立健康和医学研究所,美国Medtronic公司赞助
关键词
巴金森病
高频电刺激
细胞培养
新陈代谢
Parkinson＇s disease, high frequency electrical stimulation, cell culture, metabolism
