摘要
目的研究蒽环类化疗药物对心肌细胞的损害作用。方法体外传代培养H9c2乳鼠心肌细胞株,细胞传代第3天加入蒽环类药物柔红霉素(DNR)共培养,测定细胞上清液乳酸脱氢酶(LDH)浓度,以反映心肌细胞膜损害程度;采用四甲基偶氮唑盐微量酶反应比色(MTT)法检测心肌细胞生长抑制率,流式细胞仪检测心肌细胞凋亡率。结果①DNR1组、DNR2组、DNR3组的H9c2细胞生长抑制率分别为(24.93±1.52)%、(17.15±2.03)%和(13.48±2.26)%,3组间差异有统计学意义(P<0.01)。②DNR1、DNR2、DNR3组H9c2细胞上清液的LDH浓度分别为(77.00±6.24)、(64.3±10.50)和(5 7.67±7.24)U,均显著高于空白对照组的(37.00±6.56)U(P值均<0.01)。③DNR1、DNR2、DNR3组的H9c2细胞凋亡率分别为(15.47±2.46)%、(9.82±2.08)%和(9.43±1.63)%,均显著高于空白对照组的(4.49±2.96)%(P值均<0.05)。结论DNR能抑制心肌细胞生长,促进凋亡,同时具有对心肌细胞膜的损害作用。
Objective To investigate the anthracycline-induced injury of cardiomyocyte H9c2 cells. Methods H9c2 cells of new born rats were cultured with Daunorubicin（DNR） on the 3^rd day of passaging. To measure overall cell injury, we assayed the activity of lactate dehydrogenase（LDH） released into the medium after DNR treatment and the secretion of LDH was determined for evaluation of injury to cell membrane. The in vitro anti-proliferative effect of DNR on H9c2 cells was determined by MTT assay. Cell apoptosis rate was measured by flow cytometry. Results The inhibitory rates of H9c2 cells in DNR1 group, DNR2 group, and DNR3 groups were （24.93±1.52）%, （17. 15± 2. 03）% and（13. 48±2. 26）%, respectively, with significant difference found between the 3 groups （all P 〈 0.01）; the concentrations of LDH in the supernatants were （77. 00 ± 6. 24）, （64.3±10.50）and（57.67 ± 7.24）U, respectively, all significantly higher than that of the blank control group （37.00 ± 6.56）U, all P 〈 0.01）; the apoptosis rates of H9c2 cells in the 3 groups were（15.47 ± 2.46）%, （9.82 ± 2.08）% and （9.43 ± 1.63）%, respectively, all significantly higher than that of the blank control group （4.49 ± 2.96）%, all P 〈0.05）. Conclusion DNR can inhibit proliferation of H9c2 cells, induce cells apoptosis and impair cellular membrane.
出处
《上海医学》
CAS
CSCD
北大核心
2007年第9期696-698,F0003,共4页
Shanghai Medical Journal
