摘要
目的:探讨褪黑素对大鼠急性脊髓损伤的作用。方法:将72只Wistar成熟健康大鼠随机分为褪黑素组、甲泼尼龙琥珀酸钠组、生理盐水组、无水乙醇组,采用改良Allen′s技术(5g×10cm)以T9为中心制作急性脊髓损伤模型;损伤后10min分别予以褪黑素(100mg/kg)、甲泼尼龙琥珀酸钠(30mg/kg)、生理盐水及5%无水乙醇腹腔内注射,伤后2h、24h、72h分别观察各组BBB评分变化,取T8、T9、T103个脊髓节段,应用免疫组化对脊髓组织及P53阳性细胞进行标记,探讨褪黑素对急性脊髓损伤有无保护作用。结果:在急性脊髓损伤后,各观测时间点褪黑素组与甲泼尼龙琥珀酸钠组BBB评分均较生理盐水组及无水乙醇组明显升高(P<0.01),褪黑素组与甲泼尼龙琥珀酸钠组之间差异无显著性(P>0.05);P53阳性细胞的表达在脊髓损伤后各组均呈逐渐增高趋势,但褪黑素组与甲泼尼龙琥珀酸钠组较生理盐水组及无水乙醇组P53阳性细胞表达降低(P<0.01),褪黑素组与甲泼尼龙琥珀酸钠组之间P53阳性细胞表达差异无显著性(P>0.05),无水乙醇组与生理盐水组差异无显著性(P>0.05)。结论:褪黑素对大鼠急性脊髓损伤具有保护性治疗作用,其作用效果与甲泼尼龙琥珀酸钠相似。
Objective： To investigate the effects of melatonin in experimental spinal cord injury （SCI） in rats. Method： The Wistar 72 adult and health male rats were randomly allocated into four groups： melatonin group, methylprednisolone group, Sodium Chloride group and 5% Vehicle-treated group. The model of SCI was produced by Allen＇s method at the level of T9 in moderate degree. Melatonin （100mg/kg）, Methylprednisolone（30mg/kg）, Sodium Chloride-treated group and 5% Vehicle were given intraperitoneally ten minutes after SCI. All rats were evaluated by observing BBB score and the spinal cord of T8,T9,T10 were obtained at 2. 24.72 hours postinjury. Samples obtained from all groups were used for further study, including immunohistochemical staining and the expression of P53 to approach the effects of melatonin in experimental rats SCI. Result： Following SCI, within all monitoring time,the BBB score was found to increase in melatonin group and methylprednisolone group compared with vehicle-treated group and Sodium Chloride-treated group （P〈0.01）,The difference between melatonin group and methylprednisolone group was not statistically significant（P〉0.05 ）.The characteristics of P53 was rising in each group. In melatonin group and methylprednisolone group, the characteristics of P53 was found to decrease Compared with the vehicle-treated group and Sodium Chloride-treated group （P〈0.01）. The difference between melatonin group and methylprednisolone group was not statistically significant（P〉0.05）, the difference between vehicle-treated group and Sodium Chloride-treated group was not statistically significant （P〉0.05）. Conclusion： Melatonin has protective effect to experimental acute SCI, the effects are correspondent with methylprednisolone.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2007年第9期799-801,I0001,共4页
Chinese Journal of Rehabilitation Medicine
