摘要
目的通过对中国 HIV 感染者/艾滋病(AIDS)患者外周血单核/巨噬细胞标志抗原CD14及其早期活化分子 CD69的研究,探讨其外周血单核/巨噬细胞的功能状态与 HIV 感染者疾病进程的关系。方法采集57例未经抗病毒治疗的 HIV 感染者/AIDS 患者及32例健康人抗凝全血,运用流式细胞分析技术对 CD4^+T 淋巴细胞、CD14^+细胞及其 CD69抗原表达情况进行测定,并采用实时荧光定量 RT-PCR 技术检测患者血浆 HIV-1载量。结果 (1)HIV 感染者/AIDS 患者外周血CD14^+细胞颗粒度 SSC 值为76±16,CD69分子在 CD14^+细胞的表达率为27%±4%,均明显高于对照组(P<0.05),其中 AIDS 组高于 HIV 慢性感染(HIV)组及长期不进展(LTNP)组,HIV 组高于LTNP 组(P<0.05),LTNP 组与对照组比较差异无统计学意义;外周血 CD14^+细胞比例、CD14抗原水平与对照组相比差异无统计学意义。(2)HIV 感染者/AIDS 患者外周血 CD69分子在 CD14^+细胞的表达率与 CD4^+T 淋巴细胞绝对值呈明显负相关(r=-0.872,P<0.01),与 HIV-1 RNA 病毒载量呈显著正相关(r=0.697,P<0.01)。结论中国部分地区 HIV/AIDS 患者外周血单核/巨噬细胞的活化和吞噬功能较健康对照组有显著上升,且与疾病进展密切相关。
Objective To investigate the functional states of peripheral blood monocytes/ macrophages and their relation with the disease progression in people living with HIV/AIDS （PWHA）. Methods Peripheral blood samples were collected from 57 PWHA who didn＇t receive antivirus treatment before and 32 healthy controls in Jilin, Liaoning, and Henan provinces. Flow cytometry was used to detect the expression of the early activation molecule CD69 in the CD14^ ＋ cells. The plasma HIV RNA level was determined using real-time PCR. Results The CD69 expression rate in the CD14^＋ T cells of the PWHA group was 27%±4% , significantly higher than that of the controls （P 〈0.01 ）. The CD69 expression rate of the AIDS group was 39% ±3%, significantly higher than those of the HIV group and long-term nonprogressors （LTNP） group （26% ±3% and 20% ±4% respectively, both P 〈 0.05）, that of the HIV groups being significandy higher than that of the LTNP group too （ P 〈 0.05 ）. The side scatter value of the CD14^＋ T cells of the PWHA group was 76 ± 16, significandy higher than that of the control group （50 ± 12, P 〈 0.05 ）. The monocyte/macrophage CD69 expression rate was significantly negatively correlated with the absolute value of CD4 ^＋T cells （ r = - 0. 872 ,P 〈 0.01 ） , and not correlated with the value of CD8 ＋ T cells （ P 〉 0.05 ）. The monocyte/macrophage CD69 expression rate was significandy positively correlated with the HIV-1 RNA viral load （ r = 0. 697, P 〈 0.01 ）. Conclusion The activation and the phagocytosis function of the monocytes/macrophages of the PWHA in China are higher than those of the healthy control. They are positively correlated with the disease progression.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第34期2394-2397,共4页
National Medical Journal of China
基金
国家重点基础研究发展规划资助项目(2006CB504206)
卫生部艾滋病防治应用性研究资助项目(WA2006-02)
辽宁省重点实验室资助项目(辽科发[2005]36号)
