摘要
目的建立1型单纯疱疹病毒（herpes simplex virus type1，HSV-1）潜伏感染再激活导致小鼠面瘫的模型，观察免疫球蛋白（IgG）和干扰素对单纯疱疹病毒性小鼠面瘫的预防效果。方法64只4周龄雌性Balb／c小鼠，用26G针头搔刮小鼠耳廓背面近耳根部皮肤，左耳接种HSV．125山，总剂量1．7×10^7空斑形成单位（plaque forming unit，PFU）／ml，右耳接种25山磷酸盐缓冲液作为对照，制作小鼠面瘫潜伏模型。按数字表法将小鼠随机分为3组：第一组20只，每天腹腔注射IgG1ml／kg，连续注射3d，待出现面瘫后，面瘫小鼠继续注射3d；第二组20只，腹腔注射干扰素1．5×10’IU／kg，连续3d，待出现面瘫后，面瘫小鼠继续注射3d；第三组24只，腹腔注射相应量的生理盐水作为对照。观察小鼠面瘫情况，8周后取面瘫恢复的小鼠，腹腔注射环孢素50mg／kg，建立面瘫复发模型。处死小鼠，分离双侧面神经和三叉神经节，多聚酶链反应（polymerase chain reaction，PCR）检测HSV-1 DNA的表达。结果第一组小鼠面瘫率50％，面瘫持续时间（7．2±2．2）d（x±s，下同）；第二组小鼠面瘫率30％，面瘫持续时间（4．5±1．8）d；第三组小鼠面瘫率67％，面瘫持续时间（8．9±2．6）d。经统计学分析，IgG不能有效降低面瘫发生率及缩短面瘫持续时间（P值均〉0．05），而干扰素可以有效降低面瘫发生率并缩短面瘫持续时间（P值均〈0．05）。注射环孢素后3只面瘫恢复小鼠（3／28）再次出现面瘫，复发率11％。所有面瘫复发小鼠均检测到HSV-1DNA，而未复发小鼠均未检测出HSV-1DNA。结论HSV-1潜伏感染再激活可能是单纯疱疹病毒性小鼠面瘫复发的原因之一，潜伏病毒的再激活与免疫力低下有关。干扰素可以有效降低单纯疱疹病毒性面瘫发生率和缩短面瘫持续时间；免疫球蛋白（IgG）不能有效降低面瘫发
Objective To establish an animal model of Bell＇s palsy induced by the reactivation of latent herpes simplex virus type 1 （ HSV-1 ） , and observe the effect of interferon and IgG on the facial nerve paralysis induced by HSV-1 infection. Methods Totaly 64 four-week-old female Balb/c mice weighted 16 - 18 gram were selected . Using scratching the surface of bilateral auricles by a 26-gauge needle, 25 μl HSV-1 with a titer of 6.7×10^8 PFU/ml was inoculated into the left auricle and the same volume of PBS was placed in the right in order to develop a mouse model of latent HSV-1. In this study, interferon and IgG administration were performed before and after facial nerve paralysis and continued for 3 days. Controls were given normal sodium instead of interferon and IgG , and the incidence and duration of facial nerve paralysis were compared in the groups interferon and IgG and control. Cioclosporin was given to the mice eight weeks after recovery from facial nerve paralysis caused by inoculation with HSV-1. The HSV-1 DNA in bilateral facial nerve and bilateral trigeminal ganglion after the treatment were examined with polymerase chain reaction （PCR） analysis. Results There were 10 mice of facial nerve paralysis in the first group. The incidence of facial nerve paralysis was 50% and the duration of facial nerve paralysis was （7. 2±2. 2） days. There were 6 mice of facial nerve paralysis in the second group. The incidence of facial nerve paralysis was 30% and the duration of facial nerve paralysis was （4. 5 ±1.8） days . There were 16 mice of facial nerve paralysis in the control group. The incidence of facial nerve paralysis was 67% and the duration of facial nerve paralysis was （8.9±2.6）days. IgG didn＇t reduce the incidence and duration of facial nerve paralysis by statistics analysis （P 〉 0.05 ）, but interferon reduced the incidence and duration of facial nerve paralysis （P 〈 0. 05 ）. After administration of ciclosporin,3/28 of mice developed facial nerve paralysis. The HSV-1 DN
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2007年第9期683-686,共4页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
