摘要
目的:研究系统性红斑狼疮(SLE)患者血清IL-10与T细胞凋亡的关系。方法:采用ABC-ELISA法检测SLE患者血清IL-10含量;三色荧光流式细胞术检测患者外周血中CD3+、CD4+、CD8+T细胞亚群及其Fas、FasL的表达和早期凋亡,并分析其与血清IL-10水平的相关性。结果:SLE患者血清IL-10水平明显升高,并与SLE活动指数(SLEDAI)和血清中抗ds-DNA抗体呈正相关;外周血中T细胞CD4+亚群比例下降、CD4+/CD8+的比值降低,CD3+、CD4+、CD8+T细胞Fas、FasL的表达均增高,T细胞尤其是CD4+亚群凋亡增加,以上变化以活动性SLE更为显著;SLE患者血清IL-10水平与CD4+/CD8+细胞比值呈负相关,与T细胞FasL表达异常增加和CD4+T细胞亚群凋亡显著增多呈正相关。结论:SLE异常增高的IL-10促使T细胞高表达FasL,诱导CD4+T细胞凋亡,参与了SLE的病理进程。
Objective：To investigate the relationship between serum IL-10 and apoptosis of T lymphocytes in systemic lupus erythematosus （SLE）. Methods：ABC-ELISA was used to analyze the level of serum IL-10. Three-color flow cytometry was used to determine the changes of percentages of peripheral T lymphocyte subsets ,their expressions of Fas and FasL,and of their early apoptosis. And then, the relationship between these changes and the level of serum IL-10 was analyzed. Results：In SLE,compared with normal controls, the serum IL-10 level increased significantly,and had a positive correlation with SLEDAI and the level of serum anti-dsDNA antibodies. In peripheral blood,the percentage of CD4^＋ cells and CD4^＋/CD8^＋ ratio was decreased,and the expression rates of Fas and FasL of T lymphocyte subsets was increased. The early apeptosis of T lymphocyte subsets,especially of CD4^＋ subpopulation,was increased. These results above became more obvious in SLE patients with active disease. In SLE,there was a negative correlation between serum IL-10 level and CD4^＋/CD8^＋ ratio,while a positive correlation between serum IL-10 level and the increased expression of FasL, and the increased apoptosis of CD4^＋ T lymphocyte. Conclusion：The abnormally increased serum IL-10 level in SLE patients can induce a high level of FasL,which mediates the apoptosis of CD4^＋ T lymphocyte,and involves in a more active disease.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第10期1118-1122,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
常州市社会发展研究项目(CS2006911)
留学人员科技活动择优资助项目(苏人通[2004]137)
