摘要
目的:探讨PKC在脊髓空洞前状态中脊髓水肿中的作用。方法:用新西兰兔制作模型,术后1、3、7、14、21d用光镜、干湿重法观察上颈髓病理学演变、脊髓含水量;应用底物磷酸化法测定胞膜、胞浆PKC活性;用伊文氏蓝(Evansblue)法定量测定血脊髓屏障损害程度。结果:Kaolin组动物于术后第1d脊髓含水量即有明显增加,第7d达到高峰,持续至14d,至21d时稍有缓解;胞膜PKC活性术后1d出现增加,7～14d达到最高水平,21d开始回落,胞浆的PKC活性则呈相反趋势;EB含量于第3d脊髓即有明显增加,第7d达到高峰,并持续到21d。结论:脊髓空洞前状态形成中出现PKC移位激活,参与了缺血、缺氧性脊髓水肿的形成。
Objective： To investigate the effects of PKC on spinal edema in experimental presyrinx state in rabbits. Methods： The experimental models were established in rabbits by intra-cisternal injection of Kaolin. The water content, pathological changs, PKC activity and blood spinal cord barrier function were measured with dry-wet measure, light microscope, substrate phosphorolysis kinase assay and Evans blue technique in 1,3,7,14,21d after operation respectively. Results： Water content assay and histological observation showed that spinal cord edema in Kaolin group animals appeared at the 1st day after kaolin injection, was more prominent at 3rd day, reached its peak at 7th day, and declined slowly after 3 weeks. Membrane PKC activity increased from 1st day, reached its peak at 7th day, lasted to 14th day, and then began to drop. Kytoplasm PKC activity showed opposite tendency. Evans blue content of spinal cord began to increase at the 3rd day greatly, reached its cusp at 7 day and lasted to the 21rd day. CONCLUSION： In experimental presyrinx state, transloactional activation of PKC involves in spinal edema from ischemia.
出处
《脑与神经疾病杂志》
2007年第5期329-331,385,共4页
Journal of Brain and Nervous Diseases
基金
河北省科技厅资助项目(0620611136D-11)
河北省卫生厅资助项目(06085)
