摘要
目的运用中药血清药理学方法研究步长脑心通抗缺氧诱导的原代皮质神经元凋亡的作用。方法培养出生24h内SD乳鼠大脑皮质神经元,第7天以不同浓度步长脑心通含药血清处理后制造缺氧模型,定性定量检测细胞凋亡:MTT法观察神经元活力;Hoechst33258染色分析细胞核形态的变化;琼脂糖凝胶电泳分析DNA断裂,进一步验证和检测细胞凋亡。结果步长脑心通含药血清处理后缺氧神经元活力增加,缺氧所致神经元凋亡明显减少。结论步长脑心通对缺氧引起的皮质神经元凋亡有抑制作用。
Objective To investigate the effect of Buchang Naoxintong containing serum （BNCS） for antagonizing hypoxia-induced apoptosis of primary cultured cortical neurons with the sero-pharmacological method. Methods Primary cortical neurons from neonate rats （within 24 h） were cultured and induced into hypoxia model on the 7th day, which were then treated with different concentrations of BNCS. Cell apoptosis was detected qualitatively and quantitatively; and further verified by agarose gel electrophoresis through analyzing in- ternucleosomal DNA fragmentation of the neurons. Besides, neuron viability was tested by 3-（4,5-dimethyl-2- thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide （MTT） assay and change of nuclear morphology was observed by Hoechst 33258 staining. Results After treatment with BNCS, the viability of the hypoxia neurons improved with significantly reduced neuron apoptosis. Conclusion Buchang Naoxintong can protect cortical neurons from hypoxia-induced apoptosis.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2007年第9期835-838,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
步长脑心通
神经保护
凋亡
中药血清药理学
皮质神经元
Buchang Naoxintong
neuroprotection
apoptosis sero-pharmacology of Chinese drugs
cortical neuron
