摘要
目的分析不同产地白花丹药材中白花丹醌含量的差异。方法采用KromasilC18柱（4.6mm×200mm,5μm）,柱温：30℃,流动相：甲醇-水（65∶35）,检测波长：213nm,流速：1mL·min^-1。结果白花丹醌在0.0104-0.3328μg,峰面积与进样量具有良好的线性关系,回归方程为Y=-22.32＋9527.9X,r=0.9999,平均回收率为98.7%。结论本法简便、准确、重复性好,可用于控制白花丹药材质量。
OBJECTIVE To determine the Plumbagin in Plumbago zeylanica from different areas. METHODS A RP-HPLC method was established using a kromasil C18 column （4. 6 mm ×200 mm,5μm）. The mobile phase was methanol-water（65： 35）. The flow rate was 1 mL · min^- 1 with UV detection at 213 nm. The column temperature was 30℃. RESULTS The method displayed good linearity within the concentration ranges of 0. 010 4 -0. 332 8 μg （r =0. 999 9）. The average recovery was 98.7%. CONCLUSION This method is simple, accurate, reproducible and suitable for the determination of Plumbagin in P. zeylanica L.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第18期1429-1430,共2页
Chinese Pharmaceutical Journal
基金
四川省青年基金项目(07ZQ026-011)
四川省科技攻关项目(2005)
四川省科技厅应用基础项目(2006)
四川省教育厅教育教学改革项目(2005)
